Methods of cell culture

US8956830B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8956830-B2
Application numberUS-201313829292-A
CountryUS
Kind codeB2
Filing dateMar 14, 2013
Priority dateMar 14, 2013
Publication dateFeb 17, 2015
Grant dateFeb 17, 2015

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  1. Title

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  2. Abstract

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  5. First independent claim

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Abstract

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Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described.

First claim

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The invention claimed is: 1. A method of producing a recombinant protein preparation having target values of high mannose glycans and fucosylated glycans, the method comprising: (a) providing a mammalian cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising dimethylsulfoxide (DMSO) under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produc…

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What does patent US8956830B2 cover?
Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described.
Who is the assignee on this patent?
Momenta Pharmaceuticals Inc
What technology area does this patent fall under?
Primary CPC classification C12P21/005. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Feb 17 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).