US8946196B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8946196-B2 |
| Application number | US-201314041593-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 30, 2013 |
| Priority date | Apr 27, 2007 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Methods for the synthesis and purification of 9-amino alkyl tetracycline compounds are described.
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The invention claimed is: 1. A method of purifying an alkylaminomethyl minocycline compound, comprising: a) injecting a low pH aqueous solution of said alkylaminomethyl minocycline compound into a liquid chromatography device in a polar organic solvent gradient, and combining the product fractions; b) adjusting the pH of said product fractions to 4.0-4.5; c) washing said product fractions with a first non-polar organic solvent to form a first organic layer and a first aqueous…
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