Systems and methods for particle analysis
US-2024102986-A1 · Mar 28, 2024 · US
Tissue sample preparation and MALDI MS imaging thereof
US8945941B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945941-B2 |
| Application number | US-30212607-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 24, 2007 |
| Priority date | May 26, 2006 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presence of matrix, allowing for high resolution spatial mapping of protein, lipid, sugar, and/or nucleic acid distribution. In certain embodiments, the present invention relates to fixation with matrix of whole organisms. In certain embodiments, animals are perfused with fixation and matrix mixtures, which allows for direct mass spectrometry analysis.
First claim
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We claim: 1. A method of generating an image of a sample, wherein the image details the spatial distribution and abundance of compounds in the sample, comprising: (a) combining: a fixative selected from the group consisting of: (i) acetone; (ii) methanol; (iii) about 95% ethanol and about 5% acetic acid; and (iv) 1 part methanol:1 part ethanol:0.5 parts acetonitrile:0.5 parts aqueous solution of about 0.1% TFA; with a mass spectrometry matrix material selected from the group co…
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- What does patent US8945941B2 cover?
- Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presen…
- Who is the assignee on this patent?
- Agar Jeffrey N, Agar Nathalie Y R, Univ Brandeis, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification G01N1/30. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).