Method for culturing cells derived from the adipose tissue and uses thereof

US8945920B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8945920-B2
Application numberUS-16197507-A
CountryUS
Kind codeB2
Filing dateJan 26, 2007
Priority dateJan 26, 2006
Publication dateFeb 3, 2015
Grant dateFeb 3, 2015

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Abstract

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The invention concerns a method for culturing cells derived from the adipose tissue and in particular the stromal vascular fraction (SVF) to induce formation of cardiomyocytes, the use of the cells obtained by said culture method to reconstitute an ischemized cardiac zone, in particular following an infarction, as well as a pharmaceutical composition containing said cells. The method for obtaining cardiac cells comprises at least the following steps: a) selecting cardiomyogenic cells from the stromal vascular fraction (SVF); b) culturing the cells selected at step a) in a liquid medium optimized for expanding ex vivo the cardiomyogenic cells; c) maintaining and expanding said cells by successive passes in the liquid medium; and d) obtaining cardiac cells.

First claim

Opening claim text (preview).

The invention claimed is: 1. A method for obtaining cardiac cells, comprising at least the following steps: a) selecting cardiomyogenic cells from the crude stromal vascular fraction (SVF) of adipose tissue; b) culturing the cells selected in step a) in a liquid medium optimized for expanding the cardiomyogenic cells ex vivo, said liquid medium being selected from the group consisting of BHK21 medium containing at least fetal calf serum and β-mercaptoethanol and any other medium…

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What does patent US8945920B2 cover?
The invention concerns a method for culturing cells derived from the adipose tissue and in particular the stromal vascular fraction (SVF) to induce formation of cardiomyocytes, the use of the cells obtained by said culture method to reconstitute an ischemized cardiac zone, in particular following an infarction, as well as a pharmaceutical composition containing said cells. The method for obtain…
Who is the assignee on this patent?
Casteilla Louis, Planat-Benard Valérie, Penicaud Luc, and 2 more
What technology area does this patent fall under?
Primary CPC classification C12N5/0657. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).