METHODS AND SYSTEMS FOR FUNCTIONAL MATURATION OF iPSC AND ESC DERIVED CARDIOMYOCYTES
US-2024076619-A1 · Mar 7, 2024 · US
US8945920B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945920-B2 |
| Application number | US-16197507-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 26, 2007 |
| Priority date | Jan 26, 2006 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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The invention concerns a method for culturing cells derived from the adipose tissue and in particular the stromal vascular fraction (SVF) to induce formation of cardiomyocytes, the use of the cells obtained by said culture method to reconstitute an ischemized cardiac zone, in particular following an infarction, as well as a pharmaceutical composition containing said cells. The method for obtaining cardiac cells comprises at least the following steps: a) selecting cardiomyogenic cells from the stromal vascular fraction (SVF); b) culturing the cells selected at step a) in a liquid medium optimized for expanding ex vivo the cardiomyogenic cells; c) maintaining and expanding said cells by successive passes in the liquid medium; and d) obtaining cardiac cells.
Opening claim text (preview).
The invention claimed is: 1. A method for obtaining cardiac cells, comprising at least the following steps: a) selecting cardiomyogenic cells from the crude stromal vascular fraction (SVF) of adipose tissue; b) culturing the cells selected in step a) in a liquid medium optimized for expanding the cardiomyogenic cells ex vivo, said liquid medium being selected from the group consisting of BHK21 medium containing at least fetal calf serum and β-mercaptoethanol and any other medium…
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Human Necessities · mapped topic
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