Process of manufacture of annexin v
US-2024116982-A1 · Apr 11, 2024 · US
Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
US8945895B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945895-B2 |
| Application number | US-84795610-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 30, 2010 |
| Priority date | Jul 31, 2009 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.
First claim
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The invention claimed is: 1. A method for purifying recombinant a disintegrin-like and metallopeptidase with thrombospondin type I motif 13 (ADAMTS13) protein from a sample comprising ADAMTS13 protein and non-ADAMTS13 impurities, the method comprising the steps of: (a) chromatographically contacting the sample with hydroxyapatite under conditions that allow said ADAMTS13 protein to appear in a flow through fraction from said hydroxyapatite by said ADAMTS13 protein not binding to s…
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- What does patent US8945895B2 cover?
- Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The me…
- Who is the assignee on this patent?
- Hasslacher Meinhard, Fiedler Christian, Mayer Christa, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification C07K1/14. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).