Starch-derived clathrate-forming compositions
US-11959114-B2 · Apr 16, 2024 · US
Method of using alpha-amylase from Aspergillus clavatus for saccharification
US8945889B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945889-B2 |
| Application number | US-201313888303-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 6, 2013 |
| Priority date | May 11, 2012 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii . This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
First claim
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What is claimed is: 1. A method of saccharifying a composition comprising starch to produce a composition comprising glucose, wherein said method comprises: (i) contacting said composition comprising starch with an isolated AcAmyl or variant thereof having α-amylase activity comprising an amino acid sequence with at least 85% amino acid sequence identity to (a) residues 20-636 of SEQ ID NO:1 or (b) residues 20-497 of SEQ ID NO:1; and (ii) saccharifying said composition comprisi…
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- What does patent US8945889B2 cover?
- A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase …
- Who is the assignee on this patent?
- Danisco Us Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P19/14. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).