Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
In situ cloning from pathological tissue specimens
US8945883B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945883-B2 |
| Application number | US-201313848416-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 21, 2013 |
| Priority date | Apr 7, 2006 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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The present invention pertains to methods related to cloning nucleic acids from biological samples, particularly pathological tissue samples. This method includes hybridizing a population of oligonucleotide sequence probes comprising degenerate sequence tags to a fixed tissue, isolating the hybridized oligonucleotide sequence probes and amplifying the sequence tags in the hybridized oligonucleotide sequence probes. This method can be utilized to identify genes associated with disease and to quantitate the expression of disease-related transcripts. The method can also be used to identify truncated mRNAs.
First claim
Opening claim text (preview).
What is claimed: 1. A method of obtaining a cell or tissue of interest from a fixed biological specimen, said method comprising: (a) providing a population of oligonucleotide sequence probes, wherein each of said oligonucleotide sequence probes comprises a sequence tag flanked by a 5′-end extension sequence and a 3′-end extension sequence, wherein said sequence tag is a degenerate sequence and wherein at least one of said 5′-end extension sequence and said 3′-end extension sequenc…
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- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
- C12Q1/6841Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US8945883B2 cover?
- The present invention pertains to methods related to cloning nucleic acids from biological samples, particularly pathological tissue samples. This method includes hybridizing a population of oligonucleotide sequence probes comprising degenerate sequence tags to a fixed tissue, isolating the hybridized oligonucleotide sequence probes and amplifying the sequence tags in the hybridized oligonucleo…
- Who is the assignee on this patent?
- Univ Pennsylvania
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).