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Methods for recombinant expression of a polypeptide comprising a carbohydrate binding module using a host cell having reduced expression of a protease
US8945879B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945879-B2 |
| Application number | US-201414242644-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 1, 2014 |
| Priority date | Oct 1, 2007 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
First claim
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The invention claimed is: 1. A method for producing a polypeptide comprising two or more domains of which one domain is a carbohydrate binding module, wherein the method comprises the steps of (a) fermenting an isolated cell having reduced expression of a protein comprising an amino acid sequence which has at least 95% identity to amino acids 2 to 148 of SEQ ID NO: 5, said amino acid sequence having protease activity, wherein the cell produces said polypeptide; and (b) recoverin…
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Frequently asked questions
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- What does patent US8945879B2 cover?
- The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
- Who is the assignee on this patent?
- Novozymes As
- What technology area does this patent fall under?
- Primary CPC classification C12N9/62. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).