Auto-processing domains for polypeptide expression

Patent metadata

Field	Value
Publication number	US-8945876-B2
Application number	US-201213683869-A
Country	US
Kind code	B2
Filing date	Nov 21, 2012
Priority date	Nov 23, 2011
Publication date	Feb 3, 2015
Grant date	Feb 3, 2015

Abstract

Embodiments herein include methods and constructs that can be used to co-express two or more polypeptides of interest from a single polynucleotide encoding a precursor polypeptide. Within this precursor polypeptide can reside at least one autonomous processing unit, which can mediate release of flanking polypeptides of interest in cis. The processing unit can include an N-terminal autocatalytic cleavage domain and a C-terminal cleavage domain. Some embodiments include constructs and methods for co-expressing polypeptides without N- or C-terminal overhangs, in any cellular or extracellular location, and/or in stoichiometric ratios.

First claim

What is claimed is:

1. A method of expressing at least two separate polypeptides in a cell, the method comprising:
initiating expression in the cell of two polypeptides of interest from a single polynucleotide encoding, from 5′ to 3′,
a first polypeptide of interest;
a processing unit downstream of the first polypeptide of interest and comprising an N-terminal autocatalytic cleavage domain upstream of a C-terminal cleavage domain; and
a second polypeptide of interest downstre…

Assignees

• Univ Hawaii

Inventors

• Su Wei Wen
• Zhang Bei

Classifications

• C12N15/8201 Primary

  Chemistry & Metallurgy · mapped topic

• C12P21/00 Primary

  Chemistry & Metallurgy · mapped topic