Method for expressing and purifying protein by using csq-tag
US-2024209046-A1 · Jun 27, 2024 · US
Methods of purifying human recombinant growth and differentiation factor-5 (rhGDF-5) protein
US8945872B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945872-B2 |
| Application number | US-201313750436-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 25, 2013 |
| Priority date | Jan 25, 2013 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of purifying recombinant GDF-5 protein from a cell, the method comprising: providing a comprising an expression vector having a T5 or a Trc promoter operatively linked to a polynucleotide sequence that encodes a GDF-5 protein, and recovering the GDF-5 protein from the cell by contacting the GDF-5 protein with a substrate so as to isolate the GDF-5 protein, wherein the vector is a plasmid vector that is pGDF5-Trc or pGDF5-T5, the T5 or Trc promot…
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Chemistry & Metallurgy · mapped topic
- C07K14/475Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C07K14/51Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8945872B2 cover?
- Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed…
- Who is the assignee on this patent?
- Warsaw Orthopedic Inc
- What technology area does this patent fall under?
- Primary CPC classification C07K14/475. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).