TARGET-BASED METHOD FOR HIGH-THROUGHPUT AND SUBCLASS SPECIFIC IgG GLYCAN PROFILING IN HUMAN PLASMA
US-2024353417-A1 · Oct 24, 2024 · US
Process for producing polypeptide
US8945867B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945867-B2 |
| Application number | US-36404909-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 2, 2009 |
| Priority date | Oct 19, 1999 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.
First claim
Opening claim text (preview).
The invention claimed is: 1. A process for producing a polypeptide, comprising: (a) selecting a rat cell line that has been adapted to grow in a serum-free medium, wherein the rat cell line is adapted to grow in a serum-free medium by performing the step of inoculating rat cells into a medium at a cell density of 1×10 5 to 1×10 6 cells/ml, said medium containing added physiologically active substances consisting of bovine serum albumin, insulin and transferrin, and culturing s…
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- C07K16/00Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8945867B2 cover?
- The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immuno…
- Who is the assignee on this patent?
- Ogawa Tatsuya, Konno Yoshinobu, Akashi Naohisa, and 4 more
- What technology area does this patent fall under?
- Primary CPC classification C07K16/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).