Methods for improved DNA release from binding substrates and/or decreasing PCR inhibition in pathogen detection

Patent metadata

Field	Value
Publication number	US-8945834-B2
Application number	US-201213420150-A
Country	US
Kind code	B2
Filing date	Mar 14, 2012
Priority date	Mar 15, 2011
Publication date	Feb 3, 2015
Grant date	Feb 3, 2015

Abstract

Disclosed herein are processes for collecting nucleic acids from particulate samples. One embodiment disclosed herein relates to the use of ultrasonic energy to simultaneously shear large nucleic acid molecules and large particulates to very small sizes prior to or during a chemical binding step to a nucleic acid binding surface. Another embodiment involves crushing the nucleic acid binding surface prior to eluting the bound nucleic acid molecules to enable better wetting of the nucleic acid binding surface and easier diffusion of bound nucleic acid molecules out of the nucleic acid binding surface.

First claim

What is claimed is:

1. A process for collecting nucleic acids from a particulate sample comprising:
(a) mixing an aliquot of a particulate sample containing nucleic acids with a nucleic acid binding solution;
(b) shearing the nucleic acids in the mixture of step (a) by ultrasound;
(c) transferring the mixture of step (b) into a device comprising a chamber interior comprising a fibrous nucleic acid binding surface, the chamber interior being capable of expanding in size in at l…

Assignees

• Dennes T Joseph
• Perry Michael P
• Du Pont

Inventors

• Dennes T Joseph
• Perry Michael P

Classifications

• C12N15/1017 Primary

  Chemistry & Metallurgy · mapped topic