Methods and compositions comprising non-natural amino acids
US-9488660-B2 · Nov 8, 2016 · US
High-performing electrophoresis gels with extended shelf lives
US8945360B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8945360-B2 |
| Application number | US-69144010-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 21, 2010 |
| Priority date | Jan 27, 2009 |
| Publication date | Feb 3, 2015 |
| Grant date | Feb 3, 2015 |
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Abstract
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Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.
First claim
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What is claimed is: 1. A polyacrylamide gel comprising: a buffer selected from the group consisting of tris(hydroxymethyl)aminomethane and bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and taurine at a concentration of from about 100 mM to about 300 mM, in a gel matrix consisting of crosslinked polyacrylamide, said polyacrylamide gel having a pH of from about 6.4 to about 7.0. 2. The polyacrylamide gel of claim 1 wherein said crosslinked po…
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- C07K1/26Primary
Chemistry & Metallurgy · mapped topic
- G01N27/44747Primary
Physics · mapped topic
Cross-Sectional Technologies · mapped topic
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- What does patent US8945360B2 cover?
- Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.
- Who is the assignee on this patent?
- Petersen Shane, Panattoni Cory, Rowell Craig, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C07K1/26. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).