Method for producing hematopoietic stem cells using pyrazole compounds
US-9212348-B2 · Dec 15, 2015 · US
Stem cell culture medium and method of using said medium and the cells
US8940535B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8940535-B2 |
| Application number | US-98238104-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 4, 2004 |
| Priority date | Nov 4, 2003 |
| Publication date | Jan 27, 2015 |
| Grant date | Jan 27, 2015 |
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Abstract
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Methods and compositions are provided for isolation of proliferating cells. In particular, the methods enrich stem cells in a mixture of stem cells and non-stem cells, and in some cases the non-stem cells may be differentiated cells. The methods exploit the non-adherent property of stem cells, as opposed to the adherent property of differentiating cells, by serially passaging the suspended cells in liquid media.
First claim
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What is claimed is: 1. A method of isolating CD34+ stem cells from a plurality of bone marrow cells, comprising the steps of: culturing bone marrow cells in a first liquid cell culture medium that lacks serum, but that comprises interleukin-3, interleukin-6, stem cell factor, and beta-mercaptoethanol; successively passaging cells in suspension from the first medium to a subsequent liquid cell culture medium that lacks serum, but that comprises interleukin-3, interleukin-6, stem…
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- What does patent US8940535B2 cover?
- Methods and compositions are provided for isolation of proliferating cells. In particular, the methods enrich stem cells in a mixture of stem cells and non-stem cells, and in some cases the non-stem cells may be differentiated cells. The methods exploit the non-adherent property of stem cells, as opposed to the adherent property of differentiating cells, by serially passaging the suspended cell…
- Who is the assignee on this patent?
- Trisler G David, Bever Jr Christopher T, Goolsby James E, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0647. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 27 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).