Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
Kits for segregating target nucleic acid from mixed nucleic acid samples
US8940296B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8940296-B2 |
| Application number | US-201313958125-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 2, 2013 |
| Priority date | Jun 27, 2011 |
| Publication date | Jan 27, 2015 |
| Grant date | Jan 27, 2015 |
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Abstract
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The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic acid and/or nucleic acid from more than one prokaryotic or eukaryotic organisms.
First claim
Opening claim text (preview).
What is claimed: 1. A kit for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising: (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid; and (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid; wherein the buffer contains a Ca2+ concentration of…
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- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US8940296B2 cover?
- The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic aci…
- Who is the assignee on this patent?
- Flir Systems
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 27 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).