Compositions for the inactivation of virus replication and methods of making and using the same
US-10286084-B2 · May 14, 2019 · US
Modulation of human cytomegalovirus replication by micro-RNA 132 (miR132), micro-RNA 145 (mi145) and micro-RNA 212 (miR212)
US8933045B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8933045-B2 |
| Application number | US-201113227117-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 7, 2011 |
| Priority date | Mar 11, 2009 |
| Publication date | Jan 13, 2015 |
| Grant date | Jan 13, 2015 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention related to miR145, miR132, miR212, and the genes or gene products regulated by these miRNAs. miR145 is downregulated in cells infected with HCMV. This downregulation modulates expression of miR145 target genes, including IRS-I. Transfection of cells with a miR145 agent, such as a miR145 mimetic, reduces HCMV replication and protein expression. miR132 and miR212 are upregulated in cells infected with HCMV. This upregulation modulates expression of miR132 and miR212 target genes, including MeCP2 and RICS. Transfection of cells with a miR132 and/or a miR212 antagonist reduces HCMV replication and protein expression. Accordingly, the invention provides methods of attenuating HCMV replication by modulating, for example, miR145, miR132, and/or miR212, and targets thereof. Also provided are methods of detecting an HCMV infection, and compositions and kits useful for attenuating HCMV replication.
First claim
Opening claim text (preview).
What is claimed: 1. A method of inhibiting HCMV replication in a cell infected with HCMV, comprising contacting the cell with a miR145 agent in an amount effective to decrease the level of one or more miR145 targets, such that HCMV replication is inhibited. 2. The method of claim 1 , wherein the miR145 agent is selected from the group consisting of a miR145 mimic, a synthetic miR145 oligonucleotide, and an expression vector encoding miR145.…
Assignees
Inventors
Classifications
Chemistry & Metallurgy · mapped topic
- C12N15/1133Primary
Chemistry & Metallurgy · mapped topic
Physics · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
Patent family
Related publications grouped by family.
External sources
Next steps
Free tools are coming soon. Tell us what you want to track and we'll notify you.
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US8933045B2 cover?
- The present invention related to miR145, miR132, miR212, and the genes or gene products regulated by these miRNAs. miR145 is downregulated in cells infected with HCMV. This downregulation modulates expression of miR145 target genes, including IRS-I. Transfection of cells with a miR145 agent, such as a miR145 mimetic, reduces HCMV replication and protein expression. miR132 and miR212 are upregul…
- Who is the assignee on this patent?
- Kowalik Timothy F, Rodriguez-Gonzalez Mariluz, Lagadinos Alexander, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1133. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 13 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).