Restriction enzyme based whole genome sequencing

US8932812B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8932812-B2
Application numberUS-201013320379-A
CountryUS
Kind codeB2
Filing dateDec 16, 2010
Priority dateDec 17, 2009
Publication dateJan 13, 2015
Grant dateJan 13, 2015

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Abstract

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Method for de novo whole genome sequencing based on a (sequence-based) physical map of a DNA sample clone bank based on end-sequencing tagged adapter-ligated restriction fragments, in combination with sequencing adapter-ligated restriction fragments of the DNA sample wherein the recognition sequence of the restriction enzyme used in the generation of the physical map is identical to at least part of the recognition sequence of the restriction enzyme used in the generation of the DNA sample.

First claim

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The invention claimed is: 1. A method for the generation of sequence information from a DNA sample comprising a. obtaining a sequence-based physical map of a clone bank of the DNA sample, said physical map comprising a plurality of tags each comprising remains of at least one restriction enzyme's recognition site and its neighbouring genomic sequence in the DNA sample; and b. digesting the clone bank with the restriction enzyme of step (a) or an isoschizomer or a neoschizomer th…

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What does patent US8932812B2 cover?
Method for de novo whole genome sequencing based on a (sequence-based) physical map of a DNA sample clone bank based on end-sequencing tagged adapter-ligated restriction fragments, in combination with sequencing adapter-ligated restriction fragments of the DNA sample wherein the recognition sequence of the restriction enzyme used in the generation of the physical map is identical to at least pa…
Who is the assignee on this patent?
Hogers René Cornelis Josephus, Van Eijk Michael Josephus Theresia, Keygene Nv
What technology area does this patent fall under?
Primary CPC classification C12Q1/6869. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Jan 13 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).