Compositions and methods for accurately identifying mutations
US-2024409996-A1 · Dec 12, 2024 · US
US8932812B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8932812-B2 |
| Application number | US-201013320379-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 16, 2010 |
| Priority date | Dec 17, 2009 |
| Publication date | Jan 13, 2015 |
| Grant date | Jan 13, 2015 |
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Method for de novo whole genome sequencing based on a (sequence-based) physical map of a DNA sample clone bank based on end-sequencing tagged adapter-ligated restriction fragments, in combination with sequencing adapter-ligated restriction fragments of the DNA sample wherein the recognition sequence of the restriction enzyme used in the generation of the physical map is identical to at least part of the recognition sequence of the restriction enzyme used in the generation of the DNA sample.
Opening claim text (preview).
The invention claimed is: 1. A method for the generation of sequence information from a DNA sample comprising a. obtaining a sequence-based physical map of a clone bank of the DNA sample, said physical map comprising a plurality of tags each comprising remains of at least one restriction enzyme's recognition site and its neighbouring genomic sequence in the DNA sample; and b. digesting the clone bank with the restriction enzyme of step (a) or an isoschizomer or a neoschizomer th…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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