Apparatus for localizing single fluorescent molecules comprised in a sample using single molecule localization microscopy
US-2024393250-A1 · Nov 28, 2024 · US
US8929974B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8929974-B2 |
| Application number | US-75700010-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 8, 2010 |
| Priority date | Oct 9, 2007 |
| Publication date | Jan 6, 2015 |
| Grant date | Jan 6, 2015 |
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In a system and a method for examining an object containing a fluid liquid, the object is illuminated with measuring light and images are temporarily shortly subsequently recorded. The images are evaluated per pixel to determine perfusion data from a high frequency portion above 1 kHz and to determine further information about properties of the object from a low frequency portion below 100 Hz, such as a degree of oxygenation of hemoglobin, a concentration of hemoglobin or a concentration of ICG. This information determined by evaluation is displayed in a form of an image in superposition with a white light image of the object.
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What is claimed is: 1. A method for examining an object containing a flowing liquid, the method comprising: illuminating the object with measuring light and recording at least one sequence of first images (I k (i,j); k=1, . . . , N) of the illuminated object, wherein an exposure time for each recording of an image of the at least one sequence of first images (I k (i,j); k=1, . . . , N) is smaller than 1 ms; evaluating, with an evaluation system, the at least one sequence of firs…
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