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Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same
US8927699B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8927699-B2 |
| Application number | US-59081005-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 25, 2005 |
| Priority date | Feb 27, 2004 |
| Publication date | Jan 6, 2015 |
| Grant date | Jan 6, 2015 |
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Abstract
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A thermostable mutant DNA polymerase; a method for obtaining the thernmostable mutant DNA polymerase by identifying a thermostable mutant polypeptide exhibiting enzymatic activity, wherein the thermostable mutant polypeptide is a variant of DNA polymerase I obtained from Thermus aquaticus ; a polynucleotide, an expression vector, and a host cell encoding the thermostable mutant DNA polymerase; and a method of performing a reverse transcription polymerase chain reaction (RT-PCR) utilizing the thermostable mutant DNA polymerase, as well as a kit for facilitating the same.
First claim
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The invention claimed is: 1. An isolated or purified polynucleotide that encodes a polypeptide having at least 95% identity to residues 13-555 of SEQ ID NO: 26 and contains a mutation at position W550 of SEQ ID NO: 26, wherein said polypeptide has DNA polymerase activity, and wherein said polypeptide comprises at least one other mutation within residues 13-555 of SEQ ID NO: 26. 2. The isolated or purified polynucleotide of claim 1 that has at least one muta…
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- C07K14/43563Primary
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
Chemistry & Metallurgy · mapped topic
Cross-Sectional Technologies · mapped topic
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- What does patent US8927699B2 cover?
- A thermostable mutant DNA polymerase; a method for obtaining the thernmostable mutant DNA polymerase by identifying a thermostable mutant polypeptide exhibiting enzymatic activity, wherein the thermostable mutant polypeptide is a variant of DNA polymerase I obtained from Thermus aquaticus ; a polynucleotide, an expression vector, and a host cell encoding the thermostable mutant DNA polymerase;…
- Who is the assignee on this patent?
- Jestin Jean-Luc, Vichier-Guerre Sophie, Ferris Stephane, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C07K14/43563. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 06 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).