Method of reducing liquid volume surrounding beads

US8927296B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8927296-B2
Application numberUS-99293909-A
CountryUS
Kind codeB2
Filing dateMay 18, 2009
Priority dateApr 18, 2006
Publication dateJan 6, 2015
Grant dateJan 6, 2015

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  1. Title

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  2. Abstract

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  4. Key dates

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  5. First independent claim

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  7. Citations and related patents

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Abstract

Official abstract text for this publication.

The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.

First claim

Opening claim text (preview).

We claim: 1. A method of reducing a liquid volume surrounding one or more magnetically responsive beads, the method comprising: (a) providing a droplet comprising the one or more magnetically responsive beads; (b) exposing the one or more magnetically responsive beads in the droplet to a first region of a magnetic field; and (c) separating the droplet from the first region of the magnetic field via electrowetting, the one or more magnetically responsive beads remaining in a su…

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Frequently asked questions

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What does patent US8927296B2 cover?
The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol an…
Who is the assignee on this patent?
Sista Ramakrishna, Pamula Vamsee, Sudarsan Arjun, and 3 more
What technology area does this patent fall under?
Primary CPC classification B01L3/502792. Mapped technology areas include Operations & Transport.
When was this patent published?
Publication date Tue Jan 06 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).