Digital microfluidic devices and methods employing integrated nanostructured electrodeposited electrodes
US-2015377831-A1 · Dec 31, 2015 · US
US8927296B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8927296-B2 |
| Application number | US-99293909-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 18, 2009 |
| Priority date | Apr 18, 2006 |
| Publication date | Jan 6, 2015 |
| Grant date | Jan 6, 2015 |
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Official abstract text for this publication.
The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.
Opening claim text (preview).
We claim: 1. A method of reducing a liquid volume surrounding one or more magnetically responsive beads, the method comprising: (a) providing a droplet comprising the one or more magnetically responsive beads; (b) exposing the one or more magnetically responsive beads in the droplet to a first region of a magnetic field; and (c) separating the droplet from the first region of the magnetic field via electrowetting, the one or more magnetically responsive beads remaining in a su…
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