High Fidelity Restriction Endonucleases
US-2024352437-A1 · Oct 24, 2024 · US
US8927263B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8927263-B2 |
| Application number | US-201313953105-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 29, 2013 |
| Priority date | Jul 31, 2012 |
| Publication date | Jan 6, 2015 |
| Grant date | Jan 6, 2015 |
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A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.
Opening claim text (preview).
What is claimed is: 1. A microfluidic device for glycan analysis, comprising: a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group of a glucosylamine, wherein the reactive ester is attached to a solid support and the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glyc…
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