Derivatization of PNGase F released glycans on an HPLC chip

US8927263B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8927263-B2
Application numberUS-201313953105-A
CountryUS
Kind codeB2
Filing dateJul 29, 2013
Priority dateJul 31, 2012
Publication dateJan 6, 2015
Grant dateJan 6, 2015

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Abstract

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A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

First claim

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What is claimed is: 1. A microfluidic device for glycan analysis, comprising: a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group of a glucosylamine, wherein the reactive ester is attached to a solid support and the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glyc…

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What does patent US8927263B2 cover?
A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plural…
Who is the assignee on this patent?
Agilent Technologies Inc
What technology area does this patent fall under?
Primary CPC classification C12Q1/34. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Jan 06 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).