Enzymatic reaction device and enzymatic reaction method

Patent metadata

Field	Value
Publication number	US-2026056159-A1
Application number	US-202519378413-A
Country	US
Kind code	A1
Filing date	Nov 4, 2025
Priority date	May 16, 2023
Publication date	Feb 26, 2026
Grant date	—

Abstract

An enzymatic reaction device includes first and second electrodes and a voltage applicator that applies a voltage to the first and second electrodes. The first and second electrodes each include at least one of an enzyme or a coenzyme that causes a reaction of a target molecule in a sample, and an electrode body including at least one of the enzyme or coenzyme immobilized on its surface. The voltage application by the voltage applicator is performed in such a manner that a first period during which a voltage is applied to the first electrode so that the first electrode functions as a working electrode that causes a reaction of the target molecule, and a second period during which a voltage is applied to the second electrode so that the second electrode functions as a working electrode that causes a reaction of the target molecule, are alternately repeated.

First claim

What is claimed is:

1 . An enzymatic reaction device, comprising:
a first electrode and a second electrode each including:
at least one of an enzyme or a coenzyme that causes a reaction of a target molecule in a sample, and
an electrode body including at least one of the enzyme or the coenzyme immobilized on a surface of the electrode body; and
a voltage applicator that applies voltages to the first electrode and the second electrode, wherein the voltage applicator applies a voltage to the first electrode and the second electrode in such a manner that a first voltage application period during which a voltage is applied to the first electrode so that the first electrode functions as a working electrode that causes a reaction of the target molecule, and a second voltage application period during which a voltage is applied to the second electrode so that the second electrode functions as a working electrode that causes a reaction of the target molecule, are alternately repeated.

2 . The enzymatic reaction device according to claim 1 , wherein an oxidoreductase that oxidizes or reduces the target molecule is immobilized on the surface of the electrode body as the enzyme.

3 . The enzymatic reaction device according to claim 2 , wherein each of the first electrode and the second electrode includes:
an electron carrier that transports an electron between the electrode body and the oxidoreductase, a first linker in a form of a chain, and a second linker in a form of a chain, the second linker being longer than the first linker, wherein the electron carrier is immobilized on the surface of the electrode body with the first linker interposed therebetween, and the oxidoreductase is immobilized on the surface of the electrode body with the second linker interposed therebetween.

4 . The enzymatic reaction device according to claim 1 , wherein a length of the first voltage application period is identical to a length of the second voltage application period.

5 . A method for performing an enzymatic reaction using a first electrode and a second electrode each including at least one of an enzyme or a coenzyme that causes a reaction of a target molecule in a sample and an electrode body including at least one of the enzyme or the coenzyme immobilized on a surface of the electrode body, the method comprising:
applying a voltage to a first electrode so that the first electrode functions as a working electrode that causes a reaction of a target molecule; and applying a voltage to a second electrode so that the second electrode functions as a working electrode that causes a reaction of the target molecule, wherein the applying the voltage to the first electrode and the applying the voltage to the second electrode are alternately repeated.

6 . The method for performing an enzymatic reaction according to claim 5 , wherein the sample is food.

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Wayama Fumiya
• Hatsugai Noriyuki
• Okumura Yasuaki
• Tsuji Kiyotaka
• Ukai Kunihiro

Classifications

• G01N33/02

  Food · CPC title

• C12Q1/26

  involving oxidoreductase · CPC title

• G01N27/3276

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title

• C12Q1/005

  involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence) · CPC title

• G01N27/3273 Primary

  Devices therefor, e.g. test element readers, circuitry (details not specific to biochemical electrodes G01N33/4875) · CPC title