Modulation of transgene expression in plants

Patent metadata

Field	Value
Publication number	US-2026002168-A1
Application number	US-202519314465-A
Country	US
Kind code	A1
Filing date	Aug 29, 2025
Priority date	Oct 11, 2016
Publication date	Jan 1, 2026
Grant date	—

Abstract

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

First claim

What is claimed is:

1 .- 33 . (canceled)

34 . A method for producing a transgenic plant cell, the method comprising:
(a) providing a nucleic acid expression cassette comprising an herbicide tolerance gene, a plant promoter operably linked to the gene, and a target site for at least one sRNA molecule; and (b) transforming a plant cell with the expression cassette, wherein the plant cell expresses the sRNA.

35 . The method of claim 34 , wherein the sRNA hybridizes to mRNA transcribed from the herbicide tolerance gene and thereby reduces the expression of the herbicide tolerance gene.

36 . The method of claim 34 , wherein the sRNA is an endogenous sRNA that is expressed in the plant cell in a tissue-preferred, tissue-specific, or cell type-specific manner.

37 . The method of claim 34 , wherein the sRNA is miR1512 or miR3522.

38 . The method of claim 37 , wherein the target site is a nucleic acid having at least 99% sequence identity to a nucleic acid selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, and combinations thereof.

39 . The method of claim 34 , wherein the sRNA is encoded by a polynucleotide that is operably linked to a non-constitutive promoter in the genome of the plant cell.

40 . The method according to claim 39 , wherein the non-constitutive promoter is a tissue-preferred, tissue-specific, or cell type-specific.

41 . The method according to claim 39 , wherein the non-constitutive promoter is a tissue-specific promoter.

42 . The method according to claim 41 , wherein the tissue-specific promoter initiates transcription selectively in vegetative tissue.

43 . A plant tissue comprising a plant cell produced by the method according to claim 34 .

44 . A plant comprising the plant tissue of claim 43 .

Assignees

• Corteva Agriscience Llc

Inventors

• Bowling Andrew J
• German Marcelo Ariel
• Glancy Todd P
• Kumar Sandeep
• Pence Heather E
• Robinson Andrew E
• Sriram Shreedharan
• Wang Pohao
• Yerkes Carla N

Classifications

• C12N15/8274

  for herbicide resistance · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title

• C12N15/8223

  Vegetative tissue-specific promoters · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8218 Primary

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title