Rust disease resistance genes and use thereof

Patent metadata

Field	Value
Publication number	US-2025382628-A1
Application number	US-202318876444-A
Country	US
Kind code	A1
Filing date	Jun 7, 2023
Priority date	Jun 23, 2022
Publication date	Dec 18, 2025
Grant date	—

Abstract

The present invention relates to polynucleotides which, when expressed in a Triticeae plant cell, particularly wheat, barley or rye plant cells confer or enhance resistance or tolerance towards leaf rust disease and/or stripe rust disease, and to Triticeae plants comprising the polynucleotides that are tolerant or resistant to the rust diseases, as well as to methods of producing same and of identifying resistant plants.

First claim

1 . A Triticeae plant comprising at least one cell comprising a heterologous polynucleotide encoding a polypeptide having at least 80% identity to the amino acid sequence set forth in SEQ ID NO:1, wherein the heterologous polynucleotide is capable of conferring or enhancing tolerance and/or resistance of the Triticeae plant to at least one rust disease.

2 . (canceled)

3 . The Triticeae plant of claim 1 , wherein the heterologous polynucleotide comprises a nucleic acid sequence having at least 70% identity to the nucleic acid sequence set forth in SEQ ID NO:6 over the entire length of the polynucleotide.

4 . (canceled)

5 . (canceled)

6 . The Triticeae plant of claim 1 , wherein the heterologous polynucleotide comprises a nucleic acid sequence having at least 88% identity to the nucleic acid sequence set forth in SEQ ID NO:3 over the entire length of the polynucleotide.

7 . (canceled)

8 . (canceled)

9 . (canceled)

10 . (canceled)

11 . (canceled)

12 . (canceled)

13 . (canceled)

14 . (canceled)

15 . The Triticeae plant of claim 1 , wherein said plant shows the phenotype of enhanced tolerance and/or resistance to at least one rust disease compared to a corresponding Triticeae plant devoid of the heterologous polynucleotide.

16 . (canceled)

17 . (canceled)

18 . (canceled)

19 . (canceled)

20 . The Triticeae plant of claim 1 , wherein the rust disease is caused by a fungus of the genus Puccinia.

21 . The Triticeae plant of claim 20 , wherein the rust disease is selected from the group consisting of leaf rust disease, stripe rust disease and a combination thereof.

22 . (canceled)

23 . (canceled)

24 . (canceled)

25 . A seed of the Triticeae plant of claim 1 , wherein a Triticeae plant grown from the seed comprises at least one cell comprising a heterologous polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 80% identity to the amino acid sequence set forth in SEQ ID NO: 1, wherein the heterologous polynucleotide is capable of conferring or enhancing tolerance and/or resistance of said Triticeae plant to at least one rust disease.

26 . A method for producing a the Triticeae plant having enhanced resistance to at least one rust diseases of claim 1 , the method comprises introducing into at least one cell of a Triticeae plant susceptible to the-at least one rust diseases a heterologous polynucleotide comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence having at least 80% identity to the amino acid sequence set forth in SEQ ID NO:1, thereby producing a Triticeae plant showing a phenotype of enhanced tolerance and/or resistance to said at least one rust diseases compared to a corresponding control plant.

27 . (canceled)

28 . The method of claim 26 , wherein the control plant is lacking the heterologous polynucleotide, said control plant is susceptible to the at least one rust disease.

29 . (canceled)

30 . (canceled)

31 . (canceled)

32 . (canceled)

33 . (canceled)

34 . The method of claim 26 , wherein the rust disease is caused by a fungus of the genus Puccinia.

35 . The method of claim 34 , wherein the rust disease is selected from the group consisting of leaf rust disease, stripe rust disease and a combination thereof.

36 . (canceled)

37 . (canceled)

38 . (canceled)

39 . A method for selecting a Triticeae plant having an enhanced tolerance and/or resistance to at least one rust diseases, comprising the steps of:
a. providing a plurality of Triticeae plants of claim 1 ; and b. selecting a plant showing an enhanced resistance to the at least one rust diseases compared to a control Triticeae plant or to a pre-determined resistance score value; thereby selecting a Triticeae plant having enhanced resistance to said at least one rust diseases

40 . The method of claim 39 , wherein the control plant is lacking the heterologous polynucleotide, said control plant is susceptible to the at least one rust disease.

41 . The method of claim 39 , wherein selecting the plant resistant to a rust disease is performed by inoculating the plant with the respective fungus and selecting phenotypically resistant plant.

42 . (canceled)

43 . The method of claim 39 , wherein selecting a plant resistant to the at least one rust disease is performed by detecting the presence of the heterologous polynucleotide within the genetic material of the at least one cell of the plant, wherein said heterologous polynucleotide comprises a nucleic acid sequence having at least 88% identity to the nucleic acid sequence set forth in SEQ ID NO:3 over its entire length.

44 . (canceled)

45 . The method of claim 43 , wherein detecting the presence of the heterologous polynucleotide is performed by amplifying at least one marker by a pair comprising SEQ ID NO:14 and SEQ ID NO:15; a pair comprising SEQ ID NO:16 and SEQ ID NO:17; a pair of primers, the pair of primers is selected from the group consisting of a pair comprising SEQ ID NO:42 and SEQ ID NO:43; a pair comprising SEQ ID NO:44 and SEQ ID NO:45; a pair comprising SEQ ID NO:46 and SEQ ID NO: 47; a pair comprising SEQ ID NO:48 and SEQ ID NO:49; a pair comprising SEQ ID NO: 50 and SEQ ID NO:51; a pair comprising SEQ ID NO:52 and SEQ ID NO:53; a pair comprising SEQ ID NO:54 and SEQ ID NO:55; a pair comprising SEQ ID NO:56 and SEQ ID NO57; a pair comprising SEQ ID NO:58 and SEQ ID NO59; and a pair comprising SEQ ID NO:60 and SEQ ID NO:61.

46 . The method of claim 39 , wherein the rust disease is caused by a fungus of the genus Puccinia.

47 . The method of claim 46 , wherein the rust disease is selected from the group consisting of leaf rust disease, stripe rust disease and a combination thereof.

48 . (canceled)

49 . (canceled)

50 . (canceled)

51 . (canceled)

52 . (canceled)

53 . (canceled)

54 . (canceled)

55 . (canceled)

56 . (canceled)

57 . (canceled)

58 . (canceled)

59 . (canceled)

60 . (canceled)

61 . A DNA construct comprising an isolated polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 80% identity to the amino acid sequence set forth in SEQ ID NO: 1, further comprising at least one plant compatible expression regulatory element.

62 . The DNA construct of claim 61 , wherein the isolated polynucleotide comprises a nucleic acid sequence having at least 70% identity to the nucleic acid sequence set forth in SEQ ID NO:6 over its entire length.

63 . An isolated promoter sequence capable of deriving the expression of a polynucleotide within a cell of a Triticeae plant, the isolated promoter comprising a nucleic acid sequence having at

Assignees

• Univ Ramot

Inventors

• Sharon Amir
• Avni Raz
• Minz-Dub Anna
• Millet Eitan
• Sharma Davinder
• Kumar Rakesh

Classifications

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12N15/8209

  Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers · CPC title

• C07K14/415 Primary

  from plants · CPC title

• C12N15/8282 Primary

  for fungal resistance · CPC title