Method for removing extracellular antibiotic resistance genes in water

Patent metadata

Field	Value
Publication number	US-2025376396-A1
Application number	US-202318874043-A
Country	US
Kind code	A1
Filing date	Jun 13, 2023
Priority date	Sep 20, 2022
Publication date	Dec 11, 2025
Grant date	—

Abstract

Disclosed is a method for removing extracellular antibiotic resistance genes in water, which belongs to the field of environmental management. The method comprises adding a cell lysate, especially a cell lysate of E. coli , to water, wherein the cell lysate comprises various enzymes such as ribonucleotide exonuclease I, ribonucleotide exonuclease III, and DNA topoisomerase I, and can degrade extracellular antibiotic resistance genes. In addition, in view of the problems of poor stability of the cell lysate in an aqueous solution and the enzymes in the cell lysate being easily deactived due to complicated water ingredients, the present invention provides a cell lysate immobilized with a polyacrylamide (PAM) hydrogel, such that extracellular antibiotic resistance genes in sewage can be effectively reduced and the cell lysate can be reused by means of protecting the cell lysate.

First claim

1 . A method for removing extracellular antibiotic resistance genes in water, characterized by adding a cell lysate of bacteria to water, wherein the bacteria include one or more of E. coli, Acinetobacter sp., Pseudomonas putida and Acidithiobacillus ferrooxidans , the cell lysate contains enzymes that degrade DNA, and a temperature of the water is 28° C. to 37° C.

2 . The method for removing extracellular antibiotic resistance genes in water according to claim 1 , characterized in that the E. coli is E. coli MG1655, the Acinetobacter sp. is Acinetobacter baylyi ADP1, the Pseudomonas putida is Pseudomonas putida KT2440 and the Acidithiobacillus ferrooxidans is Acidithiobacillus ferrooxidans LX5.

3 . The method for removing extracellular antibiotic resistance genes in water according to claim 1 , characterized in that the cell lysate is immobilized by a hydrogel and then added to water.

4 . The method for removing extracellular antibiotic resistance genes in water according to claim 3 , characterized in that the hydrogel is a polyacrylamide hydrogel.

5 . The method for removing extracellular antibiotic resistance genes in water according to claim 4 , characterized in that a immobilization method of the hydrogel comprises obtaining a cell lysate, adding acrylamide, N,N′-bisacrylamide and K 2 S 2 O 8 to the cell lysate to obtain a mixture, using N 2 to aerate the mixture, and then performing a synthesis reaction of the hydrogel.

6 . The method for removing extracellular antibiotic resistance genes in water according to claim 1 , characterized in that the cell lysate is prepared by disrupting cells, wherein a method for disrupting cells is ultrasonic disruption, and conditions of the ultrasonic disruption are as follows: instrument parameters set to the power of 10% to 40%, a temperature of 0° C. to 4° C., an interval of 5.0 s to 9.9 s per running of 3.0 s to 5.0 s, and an ultrasonic time of 5 min to 10 min.

7 . A microbial agent, characterized by comprising the cell lysate according to claim 1 or the immobilized cell lysate.

8 . Use of the microbial agent according to claim 7 in the treatment of wastewater containing extracellular antibiotic resistance genes.

Assignees

• Univ Nanjing Agricultural

Inventors

• Zheng Guanyu
• Li Fangjuan
• Zhou Lixiang

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C02F3/34

  characterised by the microorganisms used · CPC title

• C02F3/341

  Consortia of bacteria · CPC title

• C12N11/087

  Acrylic polymers · CPC title

• C02F3/348

  characterised by the way or the form in which the microorganisms are added or dosed · CPC title