Cell culture device and culture vessel

Patent metadata

Field	Value
Publication number	US-2025368942-A1
Application number	US-202519247180-A
Country	US
Kind code	A1
Filing date	Jun 24, 2025
Priority date	Jan 12, 2023
Publication date	Dec 4, 2025
Grant date	—

Abstract

A cell culture device includes: a gas supply device that supplies a gas to a gas supply path that is connected to a culture vessel, wherein a culture medium and a liquid that includes a first cell are supplied to the culture vessel, the culture medium being for culturing a second cell generated from the first cell.

First claim

1 . A cell culture device comprising:
a gas supply device that supplies a gas to a gas supply path that is connected to a culture vessel, wherein a culture medium and a liquid that includes a first cell are supplied to the culture vessel, the culture medium being for culturing a second cell generated from the first cell.

2 . The cell culture device according to claim 1 , wherein
a supply path and a discharge path are connected to the culture vessel, the supply path being for supplying the culture medium and the liquid that includes the first cell to the culture vessel, the discharge path being for discharging vessel liquid present in the culture vessel, the liquid that includes the first cell is supplied to the culture vessel from a cell vessel, the culture medium is supplied to the culture vessel from a culture medium vessel, and the cell vessel, the culture medium vessel, and the culture vessel define a closed space when the cell vessel and the culture medium vessel are each connected to the supply path and the discharge path.

3 . The cell culture device according to claim 2 , wherein
one end of a gas discharge path is connected to the culture vessel, the gas discharge path being provided separately from the gas supply path, and an other end of the gas discharge path is openable.

4 . The cell culture device according to claim 3 , wherein
a supply port that is connected to the gas supply path, and a discharge port that is connected to the one end of the gas discharge path are provided, separately from each other, to the culture vessel, and the supply port and the discharge port are provided at positions that oppose each other to interpose the culture vessel.

5 . The cell culture device according to claim 2 , wherein
the gas supply path converges with a liquid supply path that supplies a liquid to the culture vessel, and the liquid supply path is a part of the supply path.

6 . The cell culture device according to claim 5 , wherein
the gas supply device sends a gas through the liquid supply path to push, out of the liquid supply path, the liquid remaining in the liquid supply path.

7 . The cell culture device according to claim 5 , wherein
the gas supply device sends a gas through a liquid discharge path to push, out of the liquid discharge path, a liquid remaining in the liquid discharge path, the liquid discharge path being for discharging the liquid from the culture vessel, and the liquid discharge path is a part of the discharge path.

8 . The cell culture device according to claim 1 , wherein
the gas is supplied to the culture vessel while the culture vessel is tilted.

9 . The cell culture device according to claim 1 , wherein
a relief valve is provided to the gas supply path.

10 . The cell culture device according to claim 1 , comprising:
a stage on which the culture vessel is arranged; a heater provided to the stage; and a temperature sensor provided to the stage, wherein output of the heater is controlled in accordance with a temperature measured by the temperature sensor.

11 . The cell culture device according to claim 10 , wherein
the output of the heater is controlled in accordance with an amount of the gas supplied to the gas supply path and the temperature measured by the temperature sensor.

12 . The cell culture device according to claim 1 , comprising a heater for gas, the heater for gas being for heating the gas.

13 . The cell culture device according to claim 12 , wherein
the heater for gas is provided to the gas supply path.

14 . The cell culture device according to claim 12 , wherein
the heater for gas is provided to the gas supply device.

15 . The cell culture device according to claim 1 , wherein
while the second cell is being cultured in the culture vessel, the gas supply device supplies the gas to the culture vessel via the gas supply path to replace a gas that is inside the culture vessel.

16 . The cell culture device according to claim 1 , wherein
the gas is a mixed gas that includes nitrogen, oxygen, and carbon dioxide.

17 . The cell culture device according to claim 1 , wherein
the culture vessel is a vessel in which the second cell generated from the first cell is cultured, and a liquid including a viral vector is supplied to the culture vessel to infect the first cell with a virus to generate the second cell.

18 . The cell culture device according to claim 1 , wherein
the first cell is a hematopoietic stem cell, the liquid that includes the first cell is blood, and the second cell is an induced pluripotent stem (IPS) cell.

19 . A culture vessel arranged in the cell culture device according to claim 1 .

20 . The culture vessel according to claim 19 , comprising:
a supply port through which the culture medium and the liquid that includes the first cell are supplied to the culture vessel, the culture medium being from a culture medium vessel, the liquid that includes the first cell being from a cell vessel; and a discharge port through which vessel liquid present in the culture vessel is discharged, wherein the supply port and the discharge port are provided separately from each other.

21 . The culture vessel according to claim 20 , wherein
the cell vessel, the culture medium vessel, and the culture vessel define a closed space when the cell vessel and the culture medium vessel are connected to the supply port.

22 . The culture vessel according to claim 20 , wherein
in a top view, a shape of the culture vessel includes two short sides that oppose each other in one direction, and two long sides that oppose each other in an other direction that intersects the one direction, the culture vessel includes: a first supply port and a second supply port that are each the supply port; and a first discharge port and a second discharge port that are each the discharge port, the first supply port is provided to one of the two short sides of the culture vessel, the first discharge port is provided to an other of the two short sides of the culture vessel, and the second supply port and the second discharge port are provided to the other of the two short sides of the culture vessel, or are separately provided to one side and an other side, respectively, of the two long sides of the culture vessel.

23 . The culture vessel according to claim 20 , wherein
a supply path is connected to the supply port, the supply path being for supplying, to the culture vessel, the culture medium and the liquid that includes the first cell, a discharge path is connected to the discharge port, the discharge path being for discharging the vessel liquid present in the culture vessel, and the supply path and the discharge path are provided separately from each other.

24 . The culture vessel according to claim 23 , wherein
a cross-sectional area of a flow path of the supply path and a cross-sectional area of a flow path of the discharge path are different from each other.

25 . The culture vessel according to claim 23 , wherein
a filter that traps the second cell is provided to the discharge path, the second cell being included in the culture medium discharged from the culture vessel, the supply path includes a culture medium supply path through which the culture medium is supplied to the culture vessel, and the culture medium is supplied to the culture medium supply path to cause the second cell trapped by the f

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Okumura Koji

Classifications

• C12M41/44

  of volume or liquid level · CPC title

• C12M35/06

  Magnetic means (C12M35/02 takes precedence) · CPC title

• C12N5/0696

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title

• C12M23/02

  Form or structure of the vessel (large containers B65D88/00) · CPC title

• C12M47/04

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title