Culture vessel and cell culture device

Patent metadata

Field	Value
Publication number	US-2025333678-A1
Application number	US-202519262322-A
Country	US
Kind code	A1
Filing date	Jul 8, 2025
Priority date	Jan 12, 2023
Publication date	Oct 30, 2025
Grant date	—

Abstract

A culture vessel is arranged in a cell culture device and holds: a second cell generated from a first cell; and a culture medium, and the culture vessel includes: a supply port through which a liquid and the culture medium are supplied to the culture vessel, the liquid including the first cell and being from a cell vessel, the culture medium being from a culture medium vessel; and a discharge port through which vessel liquid present in the culture vessel is discharged, wherein the supply port and the discharge port are provided separately from each other.

First claim

1 . A culture vessel that is arranged in a cell culture device and holds: a second cell generated from a first cell; and a culture medium, the culture vessel comprising:
a supply port through which a liquid and the culture medium are supplied to the culture vessel, the liquid including the first cell and being from a cell vessel, the culture medium being from a culture medium vessel; and a discharge port through which vessel liquid present in the culture vessel is discharged, wherein the supply port and the discharge port are provided separately from each other.

2 . The culture vessel according to claim 1 , wherein
the cell vessel, the culture medium vessel, and the culture vessel define a closed space when the cell vessel and the culture medium vessel are connected to the supply port.

3 . The culture vessel according to claim 1 , wherein
in a top view, a shape of the culture vessel includes two short sides that oppose each other in one direction, and two long sides that oppose each other in an other direction that intersects the one direction, the culture vessel includes: a first supply port and a second supply port that are each the supply port; and a first discharge port and a second discharge port that are each the discharge port, the first supply port is provided to one of the two short sides of the culture vessel, the first discharge port is provided to an other of the two short sides of the culture vessel, and the second supply port and the second discharge port are provided to the other of the two short sides of the culture vessel, or are separately provided to one side and an other side, respectively, of the two long sides of the culture vessel.

4 . The culture vessel according to claim 1 , wherein
a liquid including a viral vector is supplied to the culture vessel to infect the first cell with a virus to generate the second cell.

5 . The culture vessel according to claim 1 , wherein
the first cell is a hematopoietic stem cell, the liquid that includes the first cell is blood, and the second cell is an induced pluripotent stem (IPS) cell.

6 . The culture vessel according to claim 1 , wherein
a supply path is connected to the supply port, the supply path being for supplying, to the culture vessel, the culture medium and the liquid that includes the first cell, a discharge path is connected to the discharge port, the discharge path being for discharging the vessel liquid present in the culture vessel, and the supply path and the discharge path are provided separately from each other.

7 . The culture vessel according to claim 6 , wherein
a cross-sectional area of a flow path of the supply path and a cross-sectional area of a flow path of the discharge path are different from each other.

8 . The culture vessel according to claim 6 , wherein
a filter that traps the second cell is provided to the discharge path, the second cell being included in the culture medium discharged from the culture vessel, the supply path includes a culture medium supply path through which the culture medium is supplied to the culture vessel, and the culture medium is supplied to the culture medium supply path to cause the second cell trapped by the filter to return to the culture vessel.

9 . A cell culture device comprising:
the culture vessel according to claim 6 ; and a gas supply device that supplies a gas, wherein the gas supply device sends a gas through the supply path to cause:
the liquid that had included the first cell and remains in the supply path to return to the cell vessel or be sent to the culture vessel; or
the culture medium that remains in the supply path to return to the culture medium vessel or be sent to the culture vessel.

10 . The cell culture device according to claim 9 , wherein
the gas supply device sends a gas through the discharge path to cause:
residual liquid that remains in the discharge path to return to the culture vessel or be sent to a waste liquid vessel.

Assignees

• Panasonic Ip Man Co Ltd

Inventors

• Okumura Koji
• Nakagawa Tohru
• Usui Yukiya
• Kato Hikaru

Classifications

• C12M23/58

  Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00) · CPC title

• C12M29/18

  External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435) · CPC title

• C12M33/14 Primary

  with filters, sieves or membranes · CPC title

• C12M29/00 Primary

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title

• C12N5/10

  Cells modified by introduction of foreign genetic material · CPC title