Carrier apparatus and sequencing system

Patent metadata

Field	Value
Publication number	US-2025270623-A1
Application number	US-202519052731-A
Country	US
Kind code	A1
Filing date	Feb 13, 2025
Priority date	Feb 26, 2024
Publication date	Aug 28, 2025
Grant date	—

Abstract

The present application discloses a carrier apparatus and a sequencing system. The carrier apparatus according to the embodiments of the present disclosure includes a carrier platform, a fluid connecting assembly, and a movement mechanism. The carrier platform includes a table surface, the table surface being detachably connected to a flow cell assembly; the flow cell assembly includes a flow cell, with a fluid inlet and a fluid outlet being respectively formed at both ends of the flow cell. The fluid connecting assembly includes a manifold arranged in correspondence with the fluid inlet and the fluid outlet. The movement mechanism drives the fluid connecting assembly to move, including: when the fluid connecting assembly is located at a first position, the fluid connecting assembly and the flow cell assembly form a fluid communication through the manifold with the fluid inlet and the fluid outlet; and when the fluid connecting assembly is located at a second position, communication between the fluid connecting assembly and the flow cell assembly is cut off. In the carrier apparatus according to the embodiments of the present disclosure, the fluid connecting assembly is driven by the movement mechanism to move relative to the flow cell assembly, such that the communication between the fluid connecting assembly and the flow cell assembly can be achieved or cut off. The carrier apparatus is simple in structure and convenient to operate.

First claim

1 - 45 . (canceled)

46 . A carrier apparatus, comprising:
a carrier platform, wherein the carrier platform comprises a table surface, the table surface being detachably connected to a flow cell assembly; the flow cell assembly comprises a flow cell, with a fluid inlet and a fluid outlet being respectively formed at both ends of the flow cell; and a fluid connecting assembly and a movement mechanism, wherein the fluid connecting assembly comprises a manifold arranged in correspondence with the fluid inlet and the fluid outlet; and the movement mechanism drives the fluid connecting assembly to move, comprising: when the fluid connecting assembly is located at a first position, the fluid connecting assembly and the flow cell assembly form a fluid communication through the manifold with the fluid inlet and the fluid outlet; and when the fluid connecting assembly is located at a second position, communication between the fluid connecting assembly and the flow cell assembly is cut off.

47 . The carrier apparatus according to claim 46 , wherein the fluid connecting assembly comprises a support member, the manifold being arranged on the support member.

48 . The carrier apparatus according to claim 47 , wherein the fluid connecting assembly comprises an elastic member, wherein the elastic member is connected to the manifold, and the elastic member is configured to maintain the manifold in a tendency to move toward the flow cell.

49 . The carrier apparatus according to claim 47 , wherein the movement mechanism comprises a cam and a rotation shaft, wherein the cam is connected to the rotation shaft, and the rotation shaft is capable of driving the cam to rotate; and
the cam is further connected to the support member, and the cam, when rotating, drives the manifold on the support member to move to the first position or the second position.

50 . The carrier apparatus according to claim 49 , wherein a limiting hole is formed on the support member, the cam is arranged in the limiting hole, and the limiting hole cooperates with the cam, such that the cam, when rotating, is capable of driving the manifold on the support member to move to the first position or the second position.

51 . The carrier apparatus according to claim 50 , wherein the limiting hole is square and comprises a first inner wall and a second inner wall, wherein when the cam abuts against the first inner wall, the cam drives the manifold to move away from the flow cell; and
when the cam abuts against the second inner wall, the cam drives the manifold to move close to the flow cell.

52 . The carrier apparatus according to claim 51 , wherein the limiting hole comprises a third inner wall, wherein the third inner wall is arranged opposite the first inner wall and arranged closer to the manifold than the first inner wall; and
when the cam abuts against the third inner wall, the cam drives the manifold to move close to the flow cell.

53 . The carrier apparatus according to claim 46 , wherein adsorption groove channels are formed on the table surface of the carrier platform, the adsorption groove channels being evenly distributed on the table surface; and the carrier apparatus further comprises a negative-pressure system, the negative-pressure system communicating with the adsorption groove channels.

54 . The carrier apparatus according to claim 53 , wherein the negative-pressure system comprises:
a negative-pressure assembly, configured to generate negative pressure within the adsorption groove channels, wherein the negative-pressure assembly comprises a first pump and a first valve, the first valve being configured to switch between a first operating state and a second operating state, wherein when the first valve is in the first operating state, the first valve communicates with the first pump and the adsorption groove channels; and when the first valve is in the second operating state, communication between the first pump and the adsorption groove channels is cut off, and the first valve communicates with the adsorption groove channels and an external atmosphere.

55 . The carrier apparatus according to claim 54 , wherein the negative-pressure system further comprises a gas-water separation assembly, wherein the gas-water separation assembly communicates with the negative-pressure assembly and the adsorption groove channels, and the gas-water separation assembly is configured to separate gas and liquid in the negative-pressure system.

56 . The carrier apparatus according to claim 55 , wherein the gas-water separation assembly comprises a gas-water separator and a waste liquid tank, the gas-water separator comprising a first port, a second port, and a third port, wherein the first port of the gas-water separator communicates with the adsorption groove channels, the second port of the gas-water separator communicates with the waste liquid tank, and the third port of the gas-water separator communicates with the negative-pressure assembly.

57 . The carrier apparatus according to claim 56 , wherein the gas-water separation assembly further comprises a second pump, wherein the second pump communicates with the gas-water separator and the waste liquid tank, respectively, and the second pump is configured to drive liquid within the gas-water separator toward the waste liquid tank for conveyance.

58 . The carrier apparatus according to claim 46 , further comprising a thermostat assembly, the thermostat assembly being at least partially in contact with the carrier platform.

59 . The carrier apparatus according to claim 58 , further comprising a heat insulation plate, wherein the heat insulation plate encloses the thermostat assembly, and the heat insulation plate is connected to the carrier platform.

60 . The carrier apparatus according to claim 58 , wherein the thermostat assembly comprises a thermoelectric cooler and a heat dissipation structure, wherein the thermoelectric cooler is connected between the heat dissipation structure and the carrier platform, and the heat dissipation structure is configured to exchange heat with the thermoelectric cooler.

61 . The carrier apparatus according to claim 60 , wherein the heat dissipation structure comprises a heat dissipation cover plate and a heat dissipation water tank, wherein a heat dissipation flow channel is formed inside the heat dissipation water tank, and the heat dissipation cover plate is detachably connected to the heat dissipation water tank and covers the heat dissipation flow channel, the heat dissipation flow channel being configured to convey cooling liquid and conduct heat, and a liquid inlet hole and a liquid outlet hole that respectively communicate with the heat dissipation flow channel being formed on the heat dissipation cover plate.

62 . The carrier apparatus according to claim 61 , wherein the heat dissipation flow channel comprises a first flow channel, an intermediate flow channel, and a second flow channel, wherein the first flow channel communicates with the liquid inlet hole; the intermediate flow channel communicates with the first flow channel and is located on one side of the first flow channel; the second flow channel communicates with the intermediate flow channel and the liquid outlet hole, respectively, and the second flow channel is located on one side of the intermediate flow channel facing the first flow channel,
wherein the liquid inlet hole and the liquid outlet hole are respectively formed on a same side of the heat dissipation structure, and the first flow channel and the second flow channel are symmetrically arrange

Assignees

• Genemind Biosciences Co Ltd

Inventors

• Li Wusheng
• Wang Guangming

Classifications

• B01L2300/0877

  Flow chambers · CPC title

• B01L2400/0622

  distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves · CPC title

• B01L2400/0666

  Solenoid valves · CPC title

• B01L2300/185

  using a liquid as fluid · CPC title

• B01L2300/1838

  using fluid heat transfer medium · CPC title