Novel resistance genes associated with disease resistance in soybeans

Patent metadata

Field	Value
Publication number	US-2025243506-A1
Application number	US-202418952046-A
Country	US
Kind code	A1
Filing date	Nov 19, 2024
Priority date	Aug 1, 2019
Publication date	Jul 31, 2025
Grant date	—

Abstract

The present invention relates to methods and compositions for identifying, selecting and/or producing a Disease resistant soybean plant or germplasm using genes derived from Glycine canescens, Glycine clandestine, Glycine tomentella. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.

First claim

1 - 31 . (canceled)

32 . A nucleic acid molecule comprising a polynucleotide operably linked to a heterologous regulatory element, wherein said polynucleotide encodes a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 11, wherein co-expression of said polypeptide with a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 16, in a soybean plant, enhances resistance of the plant to Asian Soybean Rust (ASR) compared to a control plant.

33 . The nucleic acid molecule of claim 32 , wherein the polynucleotide comprises:
(a) the nucleotide sequence of any one of SEQ ID NOs: 7, or 8; or (b) a nucleotide sequence having at least 90% sequence identity to the full length of any one of SEQ ID NOs: 7 or 8.

34 . The nucleic acid molecule of claim 32 , wherein the heterologous regulatory element is a heterologous, plant expressible promoter.

35 . A vector comprising the nucleic acid molecule of claim 32 .

36 . A transgenic soybean plant cell comprising the nucleic acid molecule of claim 32 .

37 . A nucleic acid molecule comprising a polynucleotide operably linked to a heterologous regulatory element, wherein said polynucleotide encodes a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 16, wherein co-expression of said polypeptide with a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 11, in a soybean plant, enhances resistance of the plant to Asian Soybean Rust (ASR) compared to a control plant.

38 . The nucleic acid molecule of claim 37 , wherein the polynucleotide comprises:
(a) the nucleotide sequence of any one of SEQ ID NOs: 12, or 13; or (b) a nucleotide sequence having at least 90% sequence identity to the full length of any one of SEQ ID NOs: 12 or 13.

39 . The nucleic acid molecule of claim 37 , wherein the heterologous regulatory element is a heterologous, plant expressible promoter.

40 . The nucleic acid molecule of claim 39 , wherein the heterologous, plant expressible promoter comprises SEQ ID NO: 9, 14, 19, 20, 21, or 22.

41 . A vector comprising the nucleic acid molecule of claim 37 .

42 . A transgenic soybean plant cell comprising the nucleic acid molecule of claim 37 .

43 . A method of producing an Asian Soy Rust (ASR) resistant Glycine max plant, comprising the steps of:
introducing into the genome of a Glycine max plant, a heterologous nucleic acid molecule comprising:
(i) a first DNA construct comprising a first polynucleotide encoding a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 11 operably linked to a plant active promoter; and
(ii) a second DNA construct comprising a second polynucleotide encoding a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 16 operably linked to another plant active promoter,
wherein the polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 11 is co-expressed with the polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 16 in the plant, thereby producing a Glycine max plant with increased resistance to ASR.

44 . The method of claim 43 , wherein the introducing step comprises:
(a) transforming a recipient Glycine max plant cell with the heterologous nucleic acid molecule, thereby incorporating said heterologous nucleic acid molecule into the genome of the Glycine max plant cell, and regenerating a transgenic Glycine max plant from the transformed Glycine max plant cell; or (b) crossing a first Glycine max plant comprising the heterologous nucleic acid molecule with a second, different Glycine max plant.

45 . The method of claim 44 , wherein the method further comprises obtaining a progeny Glycine max plant for one or more generations from the transgenic Glycine max plant, wherein the progeny Glycine max plant comprises the heterologous nucleic acid molecule in the genome of the plant and has increased resistance to ASR.

46 . The method of claim 43 , wherein the first polynucleotide encodes a polypeptide having at least 98% sequence identity to the full length of SEQ ID NO: 11, and the second polynucleotide encodes a polypeptide having at least 98% sequence identity to the full length of SEQ ID NO: 16.

47 . The method of claim 46 , wherein the first polynucleotide encodes a polypeptide having at least 99% sequence identity to the full length of SEQ ID NO: 11, and the second polynucleotide encodes a polypeptide having at least 99% sequence identity to the full length of SEQ ID NO: 16.

48 . The method of claim 47 , wherein the first polynucleotide encodes a polypeptide comprising SEQ ID NO: 11, and the second polynucleotide encodes a polypeptide comprising SEQ ID NO: 16.

49 . A method of producing an Asian Soybean Rust (ASR) resistant Glycine max plant comprising the steps of:
a. contacting a recipient Glycine max plant cell with a heterologous nucleic acid molecule comprising a first DNA construct comprising a first polynucleotide encoding a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 11 operably linked to a plant active promoter, and a second DNA construct comprising a second polynucleotide encoding a polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 16 operably linked to another plant active promoter, wherein said heterologous nucleic acid molecule is incorporated into the genome of the recipient Glycine max plant cell, and wherein the polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 11 is co-expressed with the polypeptide having at least 95% sequence identity to the full length of SEQ ID NO: 16 in the plant cell; b. regenerating the recipient Glycine max plant cell into a transgenic Glycine max plant; and c. assaying the transgenic Glycine max plant for resistance to ASR.

50 . The method of claim 49 , wherein the first polynucleotide encodes a protein having at least 98% sequence identity to the full length of SEQ ID NO: 11, and the second polynucleotide encodes a polypeptide having at least 98% sequence identity to the full length of SEQ ID NO: 16.

51 . The method of claim 50 , wherein the first polynucleotide encodes a polypeptide having at least 99% sequence identity to the full length of SEQ ID NO: 11, and the second polynucleotide encodes a polypeptide having at least 99% sequence identity to the full length of SEQ ID NO: 16.

52 . The method of claim 51 , wherein the first polynucleotide encodes a polypeptide comprising SEQ ID NO: 11, and the second polynucleotide encodes a polypeptide comprising SEQ ID NO: 16.

53 . The method of claim 49 , wherein the contacting comprises transforming the recipient Glycine max plant cell with the heterologous nucleic acid molecule.

54 . The method of claim 49 , wherein the first polynucleotide comprises the nucleotide sequence of SEQ ID NO: 7 and the second polynucleotide comprises the nucleotide sequence of SEQ ID NO: 12.

55 . A transgenic Glycine max plant comprising in its genome a first DNA construct comprising a first polynucleotide encoding a protein having at least 95% sequence identity to the full length of SEQ ID NO: 11 operably coupled to a plant expressible promoter, and a second DNA construct comprising a second polynucleotide encoding a protein having at least 95% sequence identity to the fu

Assignees

• Syngenta Crop Protection Ag

Inventors

• Liu Qingli
• Dietrich Robert Arthur
• Curley Jr Thomas Joseph
• Breitinger Becky Welsh
• Hipskind John Daniel
• Dawson John Luther
• Tan Xiaoping
• Farmer Andrew David

Classifications

• A01H5/10

  Seeds · CPC title

• A01H1/1255

  for fungal resistance · CPC title

• A01H1/045

  using molecular markers · CPC title

• A01H6/542

  Glycine max [soybean] · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title