Electrode system and cell recording device including the same

Patent metadata

Field	Value
Publication number	US-2025222462-A1
Application number	US-202418946717-A
Country	US
Kind code	A1
Filing date	Nov 13, 2024
Priority date	Jan 10, 2024
Publication date	Jul 10, 2025
Grant date	—

Abstract

An electrode system and a cell recording device including the same are provided. The electrode system includes a multi-electrode array (MEA) layer, and a well array layer disposed to support a cell culture and disposed on the MEA layer, and wherein the well array layer further includes a degradable material.

First claim

What is claimed is:

1 . An electrode system, comprising:
a multi-electrode array (MEA) layer; and a well array layer, disposed to support a cell culture, and disposed on the MEA layer, wherein the well array layer comprises a degradable material.

2 . The electrode system of claim 1 , wherein:
a micro-sized well is disposed in the well array layer, a bottom surface of the well has at least one of a flat shape, a rounded shape, and a conical shape, and the well has a width that decreases in a downwards direction or has a predetermined width.

3 . The electrode system of claim 1 , wherein a well that is disposed in the well array layer has a width of 1 micrometer (μm) to 500 μm.

4 . The electrode system of claim 1 , wherein a well that is disposed in the well array layer has a maximum width of 100 μm to 1,000 μm, and a minimum width of 1 μm to about 1,000 μm.

5 . The electrode system of claim 1 , wherein:
the well array layer comprises a degradable polymer, a degradable hydrogel, or both, and each of the degradable polymer and the degradable hydrogel is comprised in a form of one of a fiber and a particle, or is comprised as a cross-linked network.

6 . The electrode system of claim 1 wherein the well array layer is degraded and removed by one of a mechanical factor, a physical factor, and a chemical factor.

7 . The electrode system of claim 1 , wherein the well array layer is degraded by a factor including at least one of a temperature, a light, a change in pH, a vibration, an ultrasonic wave, and an acid.

8 . The electrode system of claim 1 , wherein:
the MEA layer comprises a plurality of electrode units, a first portion of the plurality of electrode units is arranged in an area corresponding to an area of wells of the well array layer, and a second portion of the plurality of electrode units are arranged between the wells of the well array layer, and each of the electrode units comprises a single electrode or a plurality of electrodes.

9 . The electrode system of claim 1 , wherein an electrode of the MEA layer is surface-modified or surface-textured with a chemical functional group.

10 . The electrode system of claim 1 , further comprising:
an adhesion layer disposed between the well array layer and the MEA layer.

11 . The electrode system of claim 10 , wherein the adhesion layer has a thickness of 0.01 μm to 1 μm.

12 . The electrode system of claim 1 , wherein:
cells are floating-cultured into spheroids within a well that is disposed in the well array layer, the spheroids are in contact with an electrode layer corresponding to the well and are adherent-cultured, after the well is removed, and the spheroids form a connection network with each other during the adherent culturing.

13 . The electrode system of claim 12 , wherein:
the cells are nerve cells, and the connection network is formed between the spheroids through a growth of axons during the adherent culturing.

14 . The electrode system of claim 13 , wherein the electrode system is configured to measure cell information during or after the adherent culturing of the spheroids.

15 . The electrode system of claim 14 , wherein the electrode system is configured to measure an electrical signal of the nerve cells.

16 . A cell recording device, comprising:
an electrode system comprising:
a multi-electrode array (MEA) layer; and
a well array layer, disposed to support a cell culture, and disposed on the MEA layer,
wherein the well array layer comprises a degradable material.

17 . The cell recording device of claim 16 , wherein the cell recording device is configured to culture cells in vitro or in vivo, collect cell information, stimulate cells, or electroporate cells.

18 . The cell recording device of claim 16 , wherein the cell recording device is an electrophysiological system-on-a-chip (SOC) to record a signal of a nerve cell or stimulate a nerve cell.

19 . The cell recording device of claim 16 , wherein the degradable material comprises one of a degradable polymer and a degradable hydrogel.

20 . The cell recording device of claim 16 , wherein micro-sized wells are patterned on the MEA, and a spheroid network is patterned on a surface of the MEA.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Park Kitae
• Bae Chisung

Classifications

• B01L2300/163

  Biocompatibility · CPC title

• B01L2300/0645

  Electrodes · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title

• C12N5/0619

  Neurons · CPC title