Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides

Patent metadata

Field	Value
Publication number	US-2025212891-A1
Application number	US-202419000225-A
Country	US
Kind code	A1
Filing date	Dec 23, 2024
Priority date	Feb 9, 2016
Publication date	Jul 3, 2025
Grant date	—

Abstract

The present invention relates to novel mixtures comprising, as active components, at least one isolated bacterial strain, which is a member of the genus Paenibacillus, or a cell-free extract thereof or at least one metabolite thereof, and at least one chemical pesticide. The present invention also relates to compositions comprising at least one of such bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and at least one chemical pesticide. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invention also relates to mixtures of fusaricidins which are pesticidal metabolites produced by the abovementioned strains, and chemical pesticides.

First claim

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18 . A mixture, comprising as active components
1) at least one of fusaricidins 1A or 1B:

and
2) at least one pesticide II selected from selected from the groups A), B), C), D), E), G) and H):
A) Respiration inhibitors
Inhibitors of complex III at Qo site: azoxystrobin, dimoxystrobin, orysastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin;
inhibitors of complex II: benzovindiflupyr, bixafen, fluopyram, fluxapyroxad, penflufen, penthiopyrad, sedaxane, silthiofam;

B) Sterol biosynthesis inhibitors (SBI fungicides)
C14 demethylase inhibitors: difenoconazole, epoxiconazole, ipconazole, metconazole, prothioconazole, tebuconazole, triadimenol, triticonazole, 2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1-(1,2,4-triazol-1-yl) propan-2-ol, prochloraz,

C) Nucleic acid synthesis inhibitors
phenylamides or acyl amino acid fungicides: metalaxyl, metalaxyl-M;

D) Inhibitors of cell division and cytoskeleton
tubulin inhibitors: thiabendazole, thiophanate-methyl, ethaboxam;

E) Inhibitors of amino acid and protein synthesis
methionine synthesis inhibitors: pyrimethanil;

G) Lipid and membrane synthesis inhibitors
phospholipid biosynthesis and cell wall deposition: dimethomorph;

H) Inhibitors with Multi Site Action
thio- and dithiocarbamates: thiram;

in synergistically effective amounts.

19 . The mixture of claim 18 , wherein the at least one of fusaricidins 1A or 1B is a metabolite of a Paenibacillus strain selected from the group consisting of:
a) Paenibacillus strain Lu16774 deposited with DSMZ under Accession No. DSM 26969; b) Paenibacillus strain Lu17007 deposited with DSMZ under Accession No. DSM 26970; c) Paenibacillus strain Lu17015 deposited with DSMZ under Accession No. DSM 26971; and d) a bacterial strain which comprises a DNA sequence exhibiting
d1) at least 99.6% nucleotide sequence identity to the DNA sequences SEQ ID NO: 4 or SEQ ID NO:9; or
d2) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO: 14; or
d3) at least 99.9% nucleotide sequence identity to the DNA sequences SEQ ID NO: 5 or SEQ ID NO:10; or
d4) at least 99.2% nucleotide sequence identity to the DNA sequence SEQ ID NO: 15; or
d5) at least 99.2% nucleotide sequence identity to the DNA sequences SEQ ID NO: 6 or SEQ ID NO:11; or
d6) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO: 16; or
d7) at least 99.8% nucleotide sequence identity to the DNA sequences SEQ ID NO: 7 or SEQ ID NO:12; or
d8) at least 99.3% nucleotide sequence identity to the DNA sequence SEQ ID NO: 17; or
d9) 100.0% nucleotide sequence identity to the DNA sequences SEQ ID NO:8 or SEQ ID NO:13; or
d10) at least 99.9% nucleotide sequence identity to the DNA sequence SEQ ID NO:18.

20 . The mixture of claim 18 , wherein said at least one Paenibacillus strain has antifungal activity against at least two of the plant pathogen selected from the group consisting of Alternaria spp., Botrytis cinerea, Phytophthora infestans and Sclerotinia sclerotiorum.

21 . The mixture of claim 18 , wherein component 1) comprises a substantially purified culture of at least one Paenibacillus strain selected from the group consisting of:
e) Paenibacillus strain Lu16774 deposited with DSMZ under Accession No. DSM 26969; f) Paenibacillus strain Lu17007 deposited with DSMZ under Accession No. DSM 26970; g) Paenibacillus strain Lu17015 deposited with DSMZ under Accession No. DSM 26971; and h) a bacterial strain which comprises a DNA sequence exhibiting
d1) at least 99.6% nucleotide sequence identity to the DNA sequences SEQ ID NO: 4 or SEQ ID NO:9; or
d2) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO: 14; or
d3) at least 99.9% nucleotide sequence identity to the DNA sequences SEQ ID NO: 5 or SEQ ID NO:10; or
d4) at least 99.2% nucleotide sequence identity to the DNA sequence SEQ ID NO: 15; or
d5) at least 99.2% nucleotide sequence identity to the DNA sequences SEQ ID NO: 6 or SEQ ID NO:11; or
d6) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO: 16; or
d7) at least 99.8% nucleotide sequence identity to the DNA sequences SEQ ID NO: 7 or SEQ ID NO: 12; or
d8) at least 99.3% nucleotide sequence identity to the DNA sequence SEQ ID NO: 17; or
d9) 100.0% nucleotide sequence identity to the DNA sequences SEQ ID NO:8 or SEQ ID NO:13; or
d10) at least 99.9% nucleotide sequence identity to the DNA sequence SEQ ID NO:18.

22 . The mixture of claim 18 , wherein component 1) comprises a whole culture broth, a culture medium or a cell-free extract of at least one Paenibacillus strain selected from the group consisting of:
i) Paenibacillus strain Lu16774 deposited with DSMZ under Accession No. DSM 26969; j) Paenibacillus strain Lu17007 deposited with DSMZ under Accession No. DSM 26970; k) Paenibacillus strain Lu17015 deposited with DSMZ under Accession No. DSM 26971; and l) a bacterial strain which comprises a DNA sequence exhibiting
d1) at least 99.6% nucleotide sequence identity to the DNA sequences SEQ ID NO: 4 or SEQ ID NO:9; or
d2) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO: 14; or
d3) at least 99.9% nucleotide sequence identity to the DNA sequences SEQ ID NO: 5 or SEQ ID NO:10; or
d4) at least 99.2% nucleotide sequence identity to the DNA sequence SEQ ID NO: 15; or
d5) at least 99.2% nucleotide sequence identity to the DNA sequences SEQ ID NO: 6 or SEQ ID NO:11; or
d6) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO: 16; or
d7) at least 99.8% nucleotide sequence identity to the DNA sequences SEQ ID NO: 7 or SEQ ID NO:12; or
d8) at least 99.3% nucleotide sequence identity to the DNA sequence SEQ ID NO: 17; or
d9) 100.0% nucleotide sequence identity to the DNA sequences SEQ ID NO:8 or SEQ ID NO:13; or
d10) at least 99.9% nucleotide sequence identity to the DNA sequence SEQ ID NO:18.

23 . A composition comprising the mixture of claim 18 and an auxiliary.

24 . The composition of claim 23 , further comprising a pesticide III selected from the groups SF) and SI):
SF) Fungicides
inhibitors of complex III at Q o site selected from: pyraclostrobin, azoxystrobin, picoxystrobin, trifloxystrobin, dimoxystrobin, enestroburin, fenaminstrobin, fluoxastrobin, kresoxim-methyl, mandestrobine, metominostrobin, orysastrobin, pyrametostrobin, pyraoxystrobin;
broad-spectrum pyridine and pyrazole inhibitors of complex II selected from: fluxapyroxad, boscalid, benzovindiflupyr, penflufen, penthiopyrad, sedaxane, fluopyram, bixafen, isopyrazam;
Basidiomycetes-specific inhibitors of complex II selected from: carboxin, benodanil, fenfuram, flutolanil, furametpyr, mepronil, oxycarboxin, thifluzamide; ATP production inhibitor silthiofam;
fungicidal azole compounds selected from: ipconazole, difenoconazole, prothioconazole, prochloraz, triticonazole, flutriafol, cyproconazole, diniconazole, diniconazole-M, fluquinconazole, flusilazole, hexaconazole, imazalil, imibenconazole, metconaz

Assignees

• Basf Se

Inventors

• Siepe Isabella
• Liebmann Burghard
• Jabs Thorsten
• Schuster Annette

Classifications

• A01H3/00

  Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• Y02A50/30

  Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change · CPC title

• A01N37/46 Primary

  N-acyl derivatives · CPC title