Microfluidic channel backplane and microfluidic detection chip

Patent metadata

Field	Value
Publication number	US-2025170569-A1
Application number	US-202519027491-A
Country	US
Kind code	A1
Filing date	Jan 17, 2025
Priority date	Jan 23, 2020
Publication date	May 29, 2025
Grant date	—

Abstract

A microfluidic channel backplane includes a base, and a plurality of microfluidic channels, a sample-adding channel and an enrichment channel that are disposed above the base. Each microfluidic channel of the plurality of microfluidic channels includes a first end and a second end. The sample-adding channel is communicated with first ends of the plurality of microfluidic channels. The enrichment channel includes a first enrichment sub-channel and a second enrichment sub-channel. The first enrichment sub-channel is communicated with second ends of the plurality of microfluidic channels, and one end of the second enrichment sub-channel is communicated with the first enrichment sub-channel.

First claim

What is claimed is:

1 . A microfluidic channel backplane, comprising:
a base; a microfluidic channel layer disposed above the base, the microfluidic channel layer including:
a plurality of microfluidic channels, each microfluidic channel of the plurality of microfluidic channels including a first end and a second end; and
a supporting layer disposed on a side of the microfluidic channel layer away from the base, the supporting layer including:
a plurality of first supporting columns extending in a thickness direction of the base, wherein orthogonal projections of the plurality of first supporting columns on the microfluidic channel layer are located on a side of first ends of the plurality of microfluidic channels away from second ends thereof; the plurality of first supporting columns have gaps therebetween, and the gaps between the plurality of first supporting columns constitute a sample-adding channel communicated with the first ends of the plurality of microfluidic channels; the sample-adding channel is configured to add a sample to be detected into the microfluidic channel backplane; and
a plurality of second supporting columns extending in the thickness direction of the base, wherein orthogonal projections of the plurality of second supporting columns on the microfluidic channel layer are located on a side of the second ends of the plurality of microfluidic channels away from the first ends thereof; the plurality of second supporting columns have gaps therebetween, and the gaps between the plurality of second supporting columns constitute an enrichment channel communicated with the second ends of the plurality of microfluidic channels; the enrichment channel is configured to receive the sample to be detected obtained after enrichment through the plurality of microfluidic channels.

2 . The microfluidic channel backplane according to claim 1 , wherein the enrichment channel includes a first enrichment sub-channel and a second enrichment sub-channel;
the first enrichment sub-channel is communicated with the second ends of the plurality of microfluidic channels, and the second enrichment sub-channel is communicated with an end of the first enrichment sub-channel away from the second ends of the plurality of microfluidic channels.

3 . The microfluidic channel backplane according to claim 1 , wherein the sample-adding channel includes a first sample-adding sub-channel and at least two second sample-adding sub-channels, wherein
an end of the first sample-adding sub-channel is communicated with the first ends of the plurality of microfluidic channels, and another end of the first sample-adding sub-channel is communicated with an end of each second sample-adding sub-channel of the at least two second sample-adding sub-channels; a dimension of the first sample-adding sub-channel in the first direction is greater than a dimension of each second sample-adding sub-channel of the at least two second sample-adding sub-channels in the first direction.

4 . The microfluidic channel backplane according to claim 3 , wherein the at least two second sample-adding sub-channels are spaced apart and arranged in parallel in the first direction.

5 . The microfluidic channel backplane according to claim 3 , wherein the at least two second sample-adding sub-channels include two second sample-adding sub-channels;
the two second sample-adding sub-channels and the first sample-adding sub-channel are arranged in a U shape.

6 . The microfluidic channel backplane according to claim 2 , wherein
the first enrichment sub-channel extends in the first direction; and the second enrichment sub-channel extends in the second direction.

7 . The microfluidic channel backplane according to claim 6 , wherein the first enrichment sub-channel and the second enrichment sub-channel are arranged in an L shape or a T shape.

8 . The microfluidic channel backplane according to claim 1 , wherein
the supporting layer further includes a first depression and a second depression; the plurality of first supporting columns are located in the first depression, and the plurality of second supporting columns are located in the second depression, wherein the first depression and the gaps between the plurality of first supporting columns located in the first depression constitute the sample-adding channel; and the second depression and the gaps between the plurality of second supporting columns located in the second depression constitute the enrichment channel.

9 . The microfluidic channel backplane according to claim 8 , wherein the plurality of first supporting columns are uniformly arranged in the first depression, and the plurality of second supporting columns are uniformly arranged in the second depression.

10 . The microfluidic channel backplane according to claim 2 , further comprising a detection unit, wherein the detection unit is disposed proximate to the second enrichment sub-channel.

11 . The microfluidic channel backplane according to claim 1 , wherein
in the microfluidic channel layer, a first communication opening is provided on a side of the first ends of the plurality of microfluidic channels away from the second ends, and the first communication opening is configured such that the first ends of the plurality of microfluidic channels are communicated with the sample-adding channel; in the microfluidic channel layer, a second communication opening is provided on a side of the second ends of the plurality of microfluidic channels away from the first ends, and the second communication opening is configured such that the second ends of the plurality of microfluidic channels are communicated with the enrichment channel; and the first communication opening and the second communication opening extend in the first direction.

12 . The microfluidic channel backplane according to claim 1 , wherein each microfluidic channel of the plurality of microfluidic channels is of an integrally formed structure; and
the microfluidic channel backplane further comprises: a foundation layer disposed on a side of the microfluidic channel layer proximate to the base; the foundation layer including a plurality of grooves, and at least one groove of the plurality of grooves having at least one microfluidic channel of the plurality of microfluidic channels formed thereover.

13 . The microfluidic channel backplane according to claim 1 , further comprising:
a bonding film layer disposed on a side of the supporting layer away from the base; wherein the bonding film layer is configured such that the microfluidic channel backplane is bonded to a cover plate.

14 . The microfluidic channel backplane according to claim 1 , further comprising:
a control electrode disposed at a side of the plurality of microfluidic channels proximate to or away from the base, wherein an orthogonal projection of the control electrode on the base is at least partially overlapped with an orthogonal projection of each microfluidic channel of the plurality of microfluidic channels on the base; and the control electrode is configured to transmit a control signal to control an enrichment rate of microfluid flowing through the plurality of microfluidic channels.

15 . The microfluidic channel backplane according to claim 14 , wherein the control electrode extends in the first direction.

16 . The microfluidic channel backplane according to claim 1 , further comprising:
a driving electrode disposed at a side of the plurality of microfluidic channels proximate to or away from the base, wherein an orthogonal projection of the driving electrod

Assignees

• Boe Technology Group Co Ltd

Inventors

• Ma Xiaochen
• Ning ce
• Li Chao
• He Jiayu
• Zhou Xueyuan
• Zhang Xiao
• Gu Xin
• Li Zhengliang
• Yuan Guangcai

Classifications

• B01L2400/0415

  electrical forces, e.g. electrokinetic · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title

• B01L2300/0645

  Electrodes · CPC title

• B01L2300/041

  Connecting closures to device or container · CPC title