Surface capture of targets

Patent metadata

Field	Value
Publication number	US-2025163509-A1
Application number	US-202519021504-A
Country	US
Kind code	A1
Filing date	Jan 15, 2025
Priority date	Oct 10, 2018
Publication date	May 22, 2025
Grant date	—

Abstract

Provided herein are methods and systems for transfer target molecules to a surface, such as a planar surface. The transferred target molecules can be used for downstream applications, such as sequence identification.

First claim

What is claimed is:

1 . A method for analyzing nucleic acid molecules in a biological sample, comprising:
(a) providing a biological sample to an array comprising a plurality of capture probes; (b) applying a pressure field to the biological sample to direct a plurality of nucleic acid molecules in the biological sample toward the array; (c) using a subset of the plurality of capture probes to capture a subset of the plurality of nucleic acid molecules, thereby immobilizing the subset of the plurality of nucleic acid molecules to the array; (d) identifying sequences and positions of the subset of the plurality of nucleic acid molecules immobilized to the array; and (e) using the positions identified in step (d) to identify the sequences as originating from corresponding positions in the biological sample.

Assignees

• 10X Genomics Inc

Inventors

• Daugharthy Evan
• Terry Richard

Classifications

• C12Q1/6869

  Methods for sequencing · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• C12Q1/6837

  using probe arrays or probe chips (C12Q1/6874 takes precedence) · CPC title

• C12Q1/6841

  In situ hybridisation · CPC title

• C12Q1/6876 Primary

  Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title