Device for sequencing a nucleotide sequence exhibiting in crease sensitivity and im-proved reliability

Patent metadata

Field	Value
Publication number	US-2025122561-A1
Application number	US-202218720131-A
Country	US
Kind code	A1
Filing date	Dec 14, 2022
Priority date	Dec 16, 2021
Publication date	Apr 17, 2025
Grant date	—

Abstract

A device for sequencing at least one nucleotide strand including a support, at least one portion movable relative to the support, means for setting the movable portion in vibration at a given frequency, means for measuring the vibration frequency of the movable portion, and a recognition probe mechanically connecting the support and the movable portion, the recognition probe including at least one nucleotide sequence.

First claim

1 . A device for detecting at least one nucleotide strand, the device including:
a support, at least one portion movable relative to the support, means for setting the movable portion in vibration at a given frequency, means for measuring the vibration frequency of the movable portion, and a recognition probe mechanically connecting a fixed portion secured to the support and the movable portion, the recognition probe including at least one nucleotide sequence.

2 . The device according to claim 1 , wherein the means for vibrating the movable portion are configured to set the movable portion in vibration at a resonance frequency of the movable portion.

3 . The device according to claim 1 , wherein the means for measuring the vibration frequency consist of optomechanical means.

4 . The device according to claim 3 , wherein the means for measuring the vibration frequency include at least one waveguide optically coupled with the movable portion, a light source for injecting light into the waveguide and a unit for processing the light coming out of the waveguide.

5 . The device according to claim 4 , wherein the movable portion is a disk, a ring, a hippodrome, or an ellipse hanging from at least one foot.

6 . The device according to claim 1 , including several identical probes connecting the movable portion to the support.

7 . The device according to claim 1 , wherein the fixed portion includes a first adapter precursor and a first adapter, one end of which is fixed to the first adapter precursor and the other end includes a nucleotide sequence hybridised with one end of the recognition probe, and
wherein the movable portion includes a second adapter precursor and a second adapter comprising a probe, one end of which is fixed to the second adapter precursor and the other includes a nucleotide sequence hybridised with one end of the recognition probe.

8 . The device according to claim 1 , wherein the means for setting the movable portion in vibration at a given frequency are of the electrostatic type, and include one electrode on the fixed portion and one electrode on the movable portion.

9 . A set, including:
the device according to claim 1 ; and a fluid channel ensuring contact of a liquid containing said analysed strand and the recognition probe.

10 . A system including a plurality of devices according to claim 1 .

11 . The system according to claim 10 , wherein the devices are distributed in groups, each group including recognition probes having the same nucleotide structure.

12 . The system according to claim 11 , wherein the recognition probes are selected so that a whole genome is analysed in one single measurement.

13 . A detection method for sequencing at least one nucleotide sequence, the method including:
setting a liquid containing said sequence in contact with a resonating device including a recognition probe connecting a fixed portion and a movable portion of the resonating device, setting the movable portion in vibration, and measuring a variation of a vibration frequency of the movable portion due to hybridisation between the recognition probe and the nucleotide sequence.

14 . The method according claim 13 , wherein the movable portion is set in vibration at a resonance frequency of the movable portion.

15 . The method according to claim 13 , wherein the movable portion is set in vibration with an amplitude in the range of 0.1 nm and/or wherein the movable portion is set in vibration at a frequency between 100 MHz and 1 GHz.

Assignees

• Commissariat Energie Atomique

Inventors

• Alava Thomas
• Aime Jean-Pierre
• Hentz Sébastien
• Hurth Cédric

Classifications

• C12Q1/6825

  Nucleic acid detection involving sensors · CPC title

• C12Q1/6874 Primary

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title