Method for producing adipose model through environmental control and adipose model created thereby

Patent metadata

Field	Value
Publication number	US-2025109383-A1
Application number	US-202318834936-A
Country	US
Kind code	A1
Filing date	Jan 4, 2023
Priority date	Mar 8, 2022
Publication date	Apr 3, 2025
Grant date	—

Abstract

The present invention relates to a method for producing an adipose model through environmental control and an adipose model created using same, the method comprising the steps of: providing a first bioink, which is cell-unfriendly; creating a bath suspension with a predetermined volume by using the first bioink; providing a second bioink comprising preadipocytes; 3 Dprinting the second bioink in the bath suspension so as to produce an adipose model; and culturing the bath suspension in which the adipose model is disposed. The method for producing an adipose model through environmental control and the adipose model created using same, according to the present disclosure, form environments for a 3D-printed adipose model, and limit the proliferation area of adipose cells and induce the differentiation thereof, and thus can maximize similarity with actual adipose tissues.

First claim

1 . A method of producing an adipose model through environmental control, the method comprising:
preparing a cell-unfriendly first bio-ink; forming a bath suspension with the first bio-ink to have a predetermined volume; preparing a second bio-ink that contains preadipocytes; producing the adipose model by three-dimensionally printing the second bio-ink in the bath suspension; and culturing the bath suspension with the adipose model provided therein.

2 . The method of claim 1 , wherein the first bio-ink contains no cell-binding motifs.

3 . The method of claim 2 , wherein the first bio-ink contains an adipose derived extracellular matrix.

4 . The method of claim 3 , wherein the first bio-ink contains a substance to prevent the preadipocytes of the adipose model from infiltrating into the bath suspension during proliferation and differentiation.

5 . The method of claim 4 , wherein the first bio-ink contains alginate.

6 . The method of claim 5 , wherein the first bio-ink contains 1 to 2% weight/volume of the adipose derived extracellular matrix.

7 . The method of claim 6 , wherein the first bio-ink contains 1 to 2% weight/volume of the alginate.

8 . The method of claim 7 , wherein the bath suspension has shear recovery properties.

9 . The method of claim 8 , wherein the preadipocytes contained in the second bio-ink have a concentration of 1×10 6 to 1×10 8 cells/mL.

10 . An adipose model produced using an environmentally-controlled bath suspension, by performing:
preparing a cell-unfriendly first bio-ink; forming a bath suspension with the first bio-ink to have a predetermined volume; preparing a second bio-ink that contains preadipocytes; producing the adipose model by three-dimensionally printing the second bio-ink in the bath suspension; and culturing the bath suspension with the adipose model provided therein.

Assignees

• Postech Res & Business Dev Found
• Nat Univ Pusan Ind Univ Coop Found

Inventors

• Cho Dong-Woo
• Kim Byoung Soo
• Cho Won-Woo
• Ahn Min Jun

Classifications

• C12N2533/54

  Collagen; Gelatin · CPC title

• C12N2502/1305

  Adipocytes · CPC title

• C12N2501/39

  Steroid hormones · CPC title

• C12N2533/74

  Alginate · CPC title

• C12N2513/00

  3D culture · CPC title