Method for collecting microorganisms

Patent metadata

Field	Value
Publication number	US-2025059582-A1
Application number	US-202218722317-A
Country	US
Kind code	A1
Filing date	Dec 15, 2022
Priority date	Dec 22, 2021
Publication date	Feb 20, 2025
Grant date	—

Abstract

An object of the present invention is to more efficiently collect microorganisms present on a surface of an inspection object than before. A microorganism collecting method for collecting microorganisms attached to a surface of an inspection object, the method including: a liquid supply step of supplying a liquid to the surface; and a collecting step of gathering the liquid by a collection member that hardly adsorbs the microorganisms to collect the liquid.

First claim

1 . A microorganism collecting method for collecting microorganisms attached to a surface of an inspection object, the method comprising:
a liquid supply step of supplying a liquid to the surface; and a collecting step of gathering the liquid by a collection member that hardly adsorbs the microorganisms to collect the liquid from the surface.

2 . The microorganism collecting method according to claim 1 , wherein at least a portion of the collection member in contact with the liquid is formed of at least one or more substances selected from a group consisting of polyethylene, polypropylene, a silicone resin, and a thermoplastic elastomer.

3 . The microorganism collecting method according to claim 1 , wherein in the liquid supply step, the liquid is sprayed onto the surface.

4 . The microorganism collecting method according to claim 1 , further comprising a concentration step of concentrating the collected liquid.

5 . A microorganism detection method for detecting microorganisms present in the liquid collected by the microorganism collecting method according to claim 1 by a rapid microorganism test method.

6 . The microorganism detection method according to claim 5 , wherein the rapid microorganism test method measures an amount of ATP present in the liquid.

7 . The microorganism detection method according to claim 6 , wherein an ATP scavenger solution that removes free ATP is added to the liquid or a concentrate obtained by concentrating the liquid, then an ATP extraction solution is added to the liquid or the concentrate, and the amount of extracted ATP is measured.

8 . The microorganism detection method according to claim 6 , wherein a germination solution for germinating the microorganisms in a spore state is added to the liquid or a concentrate obtained by concentrating the liquid, then an ATP extraction solution is added to the liquid or the concentrate, and the amount of extracted ATP is measured.

9 . A microorganism detection system for collecting microorganisms attached to a surface of an inspection object and detecting the microorganisms, the system comprising:
a microorganism detection device that detects microorganisms contained in a liquid collected from the surface by gathering the liquid by a collection member that hardly adsorbs the microorganisms after the liquid is supplied to the surface.

Assignees

• Horiba Advanced Techno Co Ltd

Inventors

• Nakayama Hideki
• Irikura Daisuke
• Ogawa Yoko

Classifications

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title