Fluid Processing Systems Including An Electroporation Device

Patent metadata

Field	Value
Publication number	US-2025058029-A1
Application number	US-202418937842-A
Country	US
Kind code	A1
Filing date	Nov 5, 2024
Priority date	Mar 5, 2019
Publication date	Feb 20, 2025
Grant date	—

Abstract

A fluid processing system includes a separation device, a mononuclear cell product processing device, a pump assembly, and a controller. The controller is programmed to actuate the pump assembly to convey blood from a blood source into the separation device, actuate the separation device to separate a mononuclear cell product from the blood, and actuate the pump assembly to convey at least a portion of the mononuclear cell product through the mononuclear cell product processing device to modify a genome of at least one of the cells of the mononuclear cell product and create a modified mononuclear cell product. The controller is programmed to selectively actuate the pump system to convey the mononuclear cell product through the mononuclear cell product processing device in either a first direction or in an opposite, second direction to modify the genome of at least one of the cells of the mononuclear cell product.

First claim

1 - 20 . (canceled)

21 . A fluid processing system, comprising:
a separation device; a mononuclear cell product processing device; a pump assembly including a plurality of pumps; and a controller programmed to
actuate the pump assembly to convey blood from a blood source into the separation device,
actuate the separation device to separate a mononuclear cell product from the blood, and
actuate the pump assembly to convey at least a portion of the mononuclear cell product through the mononuclear cell product processing device to modify a genome of at least one of the cells of the mononuclear cell product and create a modified mononuclear cell product, wherein the controller is programmed to selectively actuate the pump system to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device in a first direction to modify the genome of said at least one of the cells of the mononuclear cell product and actuate the pump system to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device in a second direction that is opposite to the first direction to modify the genome of said at least one of the cells of the mononuclear cell product.

22 . The fluid processing system of claim 21 , wherein the separation device comprises a centrifuge.

23 . The fluid processing system of claim 21 , wherein the controller is programmed to actuate the pump system to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device in said first direction without first washing said at least a portion of the mononuclear cell product.

24 . The fluid processing system of claim 21 , wherein the controller is programmed to actuate the pump system to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device in said second direction after said at least a portion of the mononuclear cell product has been washed.

25 . The fluid processing system of claim 21 , further comprising a second separation device configured to be actuated by the controller to wash the mononuclear cell product and/or the modified mononuclear cell product.

26 . The fluid processing system of claim 25 , wherein the controller is programmed to actuate the second separation device to wash said at least a portion of the mononuclear cell product prior to actuating the pump assembly to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device.

27 . The fluid processing system of claim 25 , wherein the controller is programmed to actuate the second separation device to wash the modified mononuclear cell product after actuating the pump assembly to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device.

28 . The fluid processing system of claim 25 , wherein the controller is programmed to actuate the second separation device to wash said at least a portion of the mononuclear cell product prior to actuating the pump assembly to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device and to actuate the second separation device to wash the modified mononuclear cell product after actuating the pump assembly to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device.

29 . The fluid processing system of claim 25 , further comprising an outlet sensor associated with the second separation device and configured to detect optical characteristics of a waste product exiting the second separation device.

30 . The fluid processing system of claim 21 , wherein the controller is programmed to actuate the pump assembly to collect and retain at least a portion of the modified mononuclear cell product for a holding time prior to conveying said least a portion of the modified mononuclear cell product to the blood source.

31 . The fluid processing system of claim 21 , wherein the controller is programmed to actuate the pump assembly to convey at least a portion of the modified mononuclear cell product to the blood source without first collecting and retaining said at least a portion of the modified cell product for a holding time.

32 . The fluid processing system of claim 21 , wherein the mononuclear cell product processing device is not configured to add DNA material to said at least a portion of the mononuclear cell product.

33 . The fluid processing system of claim 21 , wherein the controller is programmed to actuate the pump assembly to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device after DNA material has been added to the mononuclear cell product.

34 . The fluid processing system of claim 21 , wherein the controller is programmed to actuate the pump assembly to add DNA material to the mononuclear cell product.

35 . The fluid processing system of claim 34 , wherein the controller is programmed to actuate the pump assembly to retain the mixture of the DNA material and the mononuclear cell product for a holding time prior to actuating the pump assembly to convey said at least a portion of the mononuclear cell product through the mononuclear cell product processing device.

36 . The fluid processing system of claim 21 , wherein the mononuclear cell product processing device comprises an electroporation device.

Assignees

• Fenwal Inc

Inventors

• Min Kyungyoon
• Kusters Benjamin E

Classifications

• A61M1/36225

  with blood pumping means or components thereof · CPC title

• A61M1/362261

  at least one cassette surface or portion thereof being flexible, e.g. the cassette having a rigid base portion with preformed channels and being covered with a foil · CPC title

• A61M1/362265

  Details of valves · CPC title

• A61M1/36224

  with sensing means or components thereof · CPC title

• A61M1/362227

  the interface providing means for actuating on functional elements of the cassette, e.g. plungers · CPC title