Activation of bioluminescence by structural complementation

Patent metadata

Field	Value
Publication number	US-2025045806-A1
Application number	US-202418893549-A
Country	US
Kind code	A1
Filing date	Sep 23, 2024
Priority date	Mar 15, 2013
Publication date	Feb 6, 2025
Grant date	—

Abstract

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

First claim

1 . A detection reagent comprising:
(a) a polypeptide comprising an amino acid sequence having less than 100% and greater than 40% sequence identity with SEQ ID NO: 440, wherein a detectable bioluminescent signal is produced in the presence of a substrate when the polypeptide contacts a peptide consisting of SEQ ID NO: 2 to form a bioluminescent complex, and (b) the substrate for the bioluminescent complex.

2 . The detection reagent of claim 1 , wherein the polypeptide comprises an amino acid sequence with at least 70% sequence identity to SEQ ID NO: 440.

3 . The detection reagent of claim 1 , wherein the polypeptide comprises an amino acid sequence selected from the polypeptide sequences of Table 2.

4 . The detection reagent of claim 2 , wherein the polypeptide comprises an amino acid sequence of SEQ ID NO: 1292.

5 . The detection reagent of claim 1 , wherein the substrate is furimazine.

6 . A detection reagent comprising:
(a) a peptide comprising an amino acid sequence having less than 100% and greater than 40% sequence identity with SEQ ID NO: 2, wherein a detectable bioluminescent signal is produced in the presence of a substrate when the peptide contacts a polypeptide consisting of SEQ ID NO: 440 to form a bioluminescent complex, and (b) the substrate for the bioluminescent complex.

7 . The detection reagent of claim 6 , wherein the peptide comprises an amino acid sequence with at least 70% sequence identity to SEQ ID NO: 2.

8 . The detection reagent of claim 6 , wherein the peptide comprises an amino acid sequence is selected from the peptide sequences of Table 1.

9 . The detection reagent of claim 8 , wherein the peptide comprises an amino acid sequence of SEQ ID NO: 390.

10 . The detection reagent of claim 8 , wherein the peptide comprises an amino acid sequence of SEQ ID NO: 2271.

11 . A detection reagent comprising:
(a) a polypeptide comprising an amino acid sequence of SEQ ID NO: 1292, and (b) furimazine.

Assignees

• Promega Corp

Inventors

• Dixon Andrew S
• Encell Lance
• Hall Mary
• Wood Keith
• Wood Monika
• Schwinn Marie
• Binkowski Brock F
• Zegzouti Hicham
• Nath Nidhi
• Mondal Subhanjan
• Goueli Said
• Meisenheimer Poncho
• Kirkland Thomas
• Unch James
• Pulukkunat Dileep K
• Robers Matthew
• Dart Melanie
• Machleidt Thomas

Classifications

• H04W4/38

  for collecting sensor information · CPC title

• H04W4/35

  for the management of goods or merchandise · CPC title

• H04W4/02

  Services making use of location information · CPC title

• G06Q30/0631

  Recommending goods or services · CPC title

• G06Q30/0283 Primary

  Price estimation or determination · CPC title