Multi-targeted, modular virus sensing platform

Patent metadata

Field	Value
Publication number	US-2025035581-A1
Application number	US-202218714512-A
Country	US
Kind code	A1
Filing date	Nov 30, 2022
Priority date	Dec 1, 2021
Publication date	Jan 30, 2025
Grant date	—

Abstract

A biosensor includes a multiplexed array of electrolyte-gated functionalized field effect transistors (FET). Walls fluidically separate each of the FETs from one another provide wells corresponding to each channel of the FETS. A perimeter wall on the upper surface of the multiplex array surrounding the separating walls and traversing the surface of all FETS in the multiplexed array. The perimeter forms a fluidically tight well traversing multiple ones of the FETs. Each of the FETs includes monolayer of a two dimensional material, such as graphene, as a conducting channel, wherein each conducting channel functionalized according to a predetermined agent.

First claim

1 . A biosensor comprising:
a multiplexed array of electrolyte-gated field effect transistors (FET); separating walls on an upper surface of the multiplexed array, the walls fluidically separating each of the FETs from one another; and a perimeter wall on the upper surface of the multiplexed array surrounding the separating walls and traversing the surface of all FETS in the multiplexed array, the perimeter wall forming a fluidically tight well traversing multiple ones of the FETs, the fluidically sealed well subdivided into a plurality of chambers, each chamber corresponding to one of the FETs in the multiplexed array;
wherein each FET comprises monolayer of a two dimensional (2D) material as a conducting channel, wherein each conducting channel functionalized according to a predetermined agent.

2 . The biosensor of claim 1 , wherein height of the separating walls is less than height of the perimeter wall.

3 . The biosensor of claim 1 , further comprising an electrolyte in the well.

4 . The biosensor of claim 3 , wherein the electrolyte is one of or a combination of phosphate buffered saline, Hanks' Balanced Salt Solution, Tris, Saliva, nasal swab dissolved in DI water or the like.

5 . The biosensor of claim 1 , wherein the separating walls comprise a polymer.

6 . The biosensor of claim 1 , wherein the separating walls comprise polydimethylsilixane, EcoFlex, SU-8, PMMA, a combination thereof or the like.

7 . The biosensor of claim 1 , wherein the separating walls comprise SiO2/Si, sapphire, glass, SiC, and flexible polyimides, parylene, polyester, tape, polycarbonate, Teflon, PET, EVA/PET.

8 . The biosensor of claim 1 , wherein the perimeter wall comprises a polymer.

9 . The biosensor of any one of claim 1 , wherein the perimeter wall comprises polydimethylsilixane, EcoFlex, SU-8, PMMA, a combination thereof or the like.

10 . The biosensor of claim 1 , wherein the perimeter wall comprises SiO2/Si, sapphire, glass, SiC, and flexible polyimides, parylene, polyester, tape, polycarbonate, Teflon, PET, EVA/PET, or the like.

11 . The biosensor of claim 1 , wherein at least one of the predetermined agents is a control.

12 . The biosensor of claim 1 , wherein at least one of the predetermined agents is flu antibody.

13 . The biosensor of claim 1 , wherein at least one of the predetermined agents is a COVID antibody.

14 . The biosensor of claim 1 , wherein at least one of the predetermined agents is an anti-ferritin antibody.

15 . The biosensor of claim 1 , wherein at least two of the FETs are functionalized differently.

16 . The biosensor of claim 1 , wherein the multiplexed array comprises an n×m array of FETs.

17 . The biosensor of claim 14 , wherein n=m.

18 . The biosensor of claim 1 , wherein the multiplexed array comprises 4 FETs in a 2×2 array.

19 . The biosensor of claim 1 , wherein the multiplexed array comprises 9 FETs in a 3×3 array.

20 . The biosensor of claim 1 , wherein the 2D material includes graphene, MoS2, hBN, WS2, WSe2, PtS2, and/or PtSe2, a combination thereof or the like.

21 - 27 . (canceled)

Assignees

• Univ Texas

Inventors

• Akinwande Deji
• Kireev Dmitry
• Kumar Neelotpala
• Towers Dalton

Classifications

• G01N2333/165

  Coronaviridae, e.g. avian infectious bronchitis virus · CPC title

• G01N2333/11

  Orthomyxoviridae, e.g. influenza virus · CPC title

• G01N33/56983

  Viruses · CPC title

• G01N33/5438

  Electrodes · CPC title

• G01N27/4145 Primary

  specially adapted for biomolecules, e.g. gate electrode with immobilised receptors · CPC title