Kits, microfluidics devices, and methods for performing biotin assays

Patent metadata

Field	Value
Publication number	US-2025027947-A1
Application number	US-202418910310-A
Country	US
Kind code	A1
Filing date	Oct 9, 2024
Priority date	Jul 30, 2018
Publication date	Jan 23, 2025
Grant date	—

Abstract

Kits containing a chemiluminescent detection system and microfluidics devices and methods for determining the concentration of biotin in a sample are disclosed. The kits, microfluidics devices, and methods utilize a sensitizer capable of generating singlet oxygen in its excited state and having avidin or an analog thereof directly or indirectly bound thereto, as well as a singlet oxygen-activatable chemiluminescent compound having biotin or an analog thereof directly or indirectly bound thereto.

First claim

What is claimed is:

1 . A kit containing a chemiluminescent detection system for determining the concentration of biotin in a sample, the kit comprising:
(a) a composition comprising a singlet oxygen-activatable chemiluminescent compound having a biotin analog directly or indirectly bound thereto; and (b) a composition comprising a sensitizer capable of generating singlet oxygen in its excited state and having avidin or an analog thereof directly or indirectly bound thereto, wherein the avidin or analog thereof is capable of binding to biotin in the sample or to (a).

2 . The kit of claim 1 , wherein the biotin analog is selected from the group consisting of 4′-hydroxyazobenzene-2-carboxylic acid (HABA), iminobiotin, biotin carbonate, biotin carbamate, biotin methyl ester, desthiobiotin, diaminobiotin, chloroacetylated biotin, biotin sulfone, thiobiotin, methoxycarbonylbiotin methyl ester, bis-biotin, tetra-biotin, and any ester, salt, or derivative of any of the above analogs.

3 . The kit of claim 2 , wherein the biotin analog is HABA or iminobiotin.

4 . The kit of claim 1 , wherein the composition of (b) has an avidin analog directly or indirectly bound to the sensitizer, and wherein the avidin analog is selected from the group consisting of avidin, streptavidin, traptavidin, neutral avidin, Neutralite avidin, Neutravidin, Lite-avidin, succinylated avidin, other forms of modified or genetically engineered avidin, and any ester, salt, or derivative of any of the above analogs.

5 . The kit of claim 1 , wherein the singlet oxygen-activatable chemiluminescent compound is a substance that undergoes a chemical reaction with singlet oxygen to form a metastabile intermediate species that can decompose with the simultaneous or subsequent emission of light.

6 . The kit of claim 1 , wherein the composition comprising the chemiluminescent compound further comprises at least one fluorescent molecule that is excited by the activated chemiluminescent compound.

7 . The kit of claim 1 , wherein the sensitizer is a photosensitizer.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Wei Tie Q
• Teng Zhu
• Liu Xiaodong
• Drinan Martin

Classifications

• B01L2200/027

  for microfluidic devices · CPC title

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• C09K11/07

  having chemically-interreactive components, e.g. reactive chemiluminescent compositions · CPC title

• F21K2/06 Primary

  using chemiluminescence · CPC title