Method for preparing pyrrolidone
US-2024132925-A1 · Apr 25, 2024 · US
Nucleic Acid Synthesis and Sequencing Using Tethered Nucleoside Triphosphates
US2025027130A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2025027130-A1 |
| Application number | US-202418804140-A |
| Country | US |
| Kind code | A1 |
| Filing date | Aug 14, 2024 |
| Priority date | Feb 27, 2022 |
| Publication date | Jan 23, 2025 |
| Grant date | — |
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Abstract
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Methods to generate novel acyl-tRNA species deploy an orthogonal synthetase that accepts α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids, and/or α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products.
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1 . A method to generate novel acyl-tRNA species, comprising reacting an orthogonal synthetase with a tRNA and a non-L-α-amino acid selected from α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids and α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products, to generate the novel acyl-tRNA species. 2 . The method of claim 1 , wherein the orthogonal synthetase accepts α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids, and α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products. 3 . The method of claim 1 , wherein the orthogonal synthetase is a pyrrolysyl-tRNA synthetase (PylRS). 4 . The method of claim 1 , wherein the orthogonal synthetase is a pyrrolysyl-tRNA synthetase (PylRS), and the PylRS is a Methanomethylophilus alvus PylRS (MaPylRS) or a MaPylRS substitution variant. 5 . The method of claim 1 , wherein the orthogonal synthetase is a pyrrolysyl-tRNA synthetase (PylRS), and the PylRS is a Methanomethylophilus alvus PylRS (MaPylRS) substitution variant comprising substitutions at N166 and V168. 6 . The method of claim 1 , wherein the orthogonal synthetase is a pyrrolysyl-tRNA synthetase (PylRS), and the PylRS is a Methanomethylophilus alvus PylRS (MaPylRS) substitution variant comprising MaFRS1 (N166A, V168L), MaFRS2 (N166A, V168K), or MaFRSA (N166A, V168A). 7 . The method of claim 1 , further comprising providing the acyl-tRNA species in a translation system, wherein the non-L-α-amino acid is incorporated into a protein. 8 . The method of claim 1 , further comprising providing the acyl-tRNA species in a translation system, wherein the non-L-α-amino acid is incorporated into a sequence-defined non-protein hetero-polymer. 9 . A composition or kit comprising an isolated orthogonal synthetase that accepts α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids or α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products. 10 . The composition or kit of claim 9 , wherein the orthogonal synthetase accepts α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids, and α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products. 11 . The composition or kit of claim 10 , wherein the orthogonal synthetase is a pyrrolysyl-tRNA synthetase (PylRS). 12 . The composition or kit of claim 11 , wherein the PylRS is a Methanomethylophilus alvus PylRS (MaPylRS) or a MaPylRS variant. 13 . The composition or kit of claim 12 , wherein the PylRS is MaPylRS variant comprising substitutions at N166 and V168. 14 . The composition or kit of claim 13 , wherein the PylRS is MaPylRS variant MaFRS1 (N166A, V168L), MaFRS2 (N166A, V168K), or MaFRSA (N166A, V168A).
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- What does patent US2025027130A1 cover?
- Methods to generate novel acyl-tRNA species deploy an orthogonal synthetase that accepts α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids, and/or α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products.
- Who is the assignee on this patent?
- Univ California
- What technology area does this patent fall under?
- Primary CPC classification C12N9/93. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jan 23 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).