Method for inhibiting biogenic hydrogen sulfide in oilfields

Patent metadata

Field	Value
Publication number	US-2025026976-A1
Application number	US-202318713690-A
Country	US
Kind code	A1
Filing date	Jun 7, 2023
Priority date	Jun 23, 2022
Publication date	Jan 23, 2025
Grant date	—

Abstract

The present invention relates to a method for inhibiting biogenic hydrogen sulfide in oilfields. A synergistic inhibition system combination agent containing Geobacillus strain GW1 is added into oilfield systems, wherein the strain GW1 has been deposited in China General Microbiological Culture Collection Center on Oct. 20, 2021, and has the deposit number of CGMCC No. 23631.

First claim

1 . A method for inhibiting biogenic hydrogen sulfide in oilfields, comprising adding a synergistic inhibition system containing strain GW1 into oilfield systems;
wherein the strain GW1 has been deposited in China General Microbiological Culture Collection Center on Oct. 20, 2021 with deposit number of CGMCC No. 23631, the synergistic inhibition system further comprises an activator and a synergistic inhibitor, the activator is sodium nitrate, and the synergistic inhibitor is barium chloride.

2 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 1 , wherein the strain GW1 is obtained by screening and culturing from production water of the oilfields.

3 . (canceled)

4 . (canceled)

5 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 2 , wherein the screening process of the strain GW1 includes:
taking the production water of oilfields as an inoculation source; carrying out the strain isolation by using anaerobic roll-tube method of Hungate; incubating anaerobic tubes in a 55° C. oven away from light for 2 weeks; and picking individual colonies and identifying.

6 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 2 , wherein conditions of the culturing of the strain GW1 comprises:
culture medium comprising: 5.00 g/L of NaCl, 0.28 g/L of MgCl 2 ·6H 2 O, 0.55 g/L of CaCl 2 ), 0.24 g/L of NH 4 Cl, 0.10 g/L of KCl, 1.50 g/L of sodium acetate, 0.85 g/L of NaNO 3 , 0.20 g/L of NaHCO 3 , 0.20 g/L of KH 2 PO 4 and 1.0 mL/L of trace elements; wherein a suitable growth temperature is 30° C.-60° C.

7 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 6 , wherein the trace elements comprise the following components:
1.20 g/L of 25% HCl, 0.20 g/L of NiCl 2 ·6H 2 O, 0.10 g/L of CaCl 2 ·2H 2 O, 1.50 g/L of FeCl 2 ·4H 2 O, 0.50 g/L of MnCl 2 ·4H 2 O, 0.02 g/L of H 3 BO 3 , 0.01 g/L of Na 2 MoO 4 ·2H 2 O, 0.50 g/L of CoCl 2 ·6H 2 O, 0.50 g/L of ZnCl 2 and 0.01 g/L of AlCl 3 .

8 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 1 , wherein the process of adding the synergistic inhibition system containing the strain GW1 into the oilfield systems is:
adding the strain GW1 and the activator sodium nitrate to a target location inhabited by sulfate-reducing microorganisms; and further adding barium chloride as the synergistic inhibitor; wherein inoculation amount of the strain GW1 transferred to the target location is 5%-9%.

9 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 8 , wherein the added amount of the activator is 0.30-0.80 g/L, and the added amount of the synergistic inhibitor is 1.50-3.00 g/L.

10 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 9 , wherein a concentration ratio of barium chloride to sulfate ions in the water sample, C barium chloride :C sulfate ions =1:1-2:1, or a concentration of barium chloride is 1.50 g/L-3.00 g/L.

11 . The method for inhibiting biogenic hydrogen sulfide in oilfields according to claim 6 , wherein an optimal growth temperature of the strain GW1 is 50° C.-55° C.

Assignees

• Univ East China Science & Tech

Inventors

• Mu Bozhong
• Zhou Lei
• Liu Yifan
• Yang Shizhong
• Wu Jun

Classifications

• C09K2208/20

  Hydrogen sulfide elimination · CPC title

• C09K8/54 Primary

  Compositions for in situ inhibition of corrosion in boreholes or wells · CPC title

• C09K8/582

  characterised by the use of bacteria · CPC title

• C12R2001/07

  Bacillus · CPC title

• C09K8/532

  Sulfur · CPC title