Methods of increasing the expression yield of vitamin K-dependent proteins
US-9212214-B2 · Dec 15, 2015 · US
Cell Growth Promoter and Application thereof
US2025019646A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2025019646-A1 |
| Application number | US-202418668297-A |
| Country | US |
| Kind code | A1 |
| Filing date | May 20, 2024 |
| Priority date | Jul 12, 2023 |
| Publication date | Jan 16, 2025 |
| Grant date | — |
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- Title
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Abstract
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A cell growth promoter and an application thereof are provided. The invention adopts Fe 2+ and ovalbumin to form a Fe 2+ -ovalbumin (OVA) compound to promote ovalbumin cell endocytosis, the cell growth promoter can maintain cell growth in an environment free of amino acids, promote cell proliferation in an environment with amino acids; and, the Fe 2+ -OVA compound can replace expensive animal serum albumin in cell culture, the cell growth promoter has a significant effect on improving cell viability and is low cost.
First claim
Opening claim text (preview).
What is claimed is: 1 . A cell culture medium, comprising a Fe 2+ -ovalbumin (OVA) compound, wherein a concentration of the Fe 2+ -OVA compound in the cell culture medium is 1 μg/mL-1000 μg/mL; the cell culture medium does not contain an amino acid; and the cell culture medium does not contain an animal serum albumin. 2 . A cell culture method, comprising culturing cells in the cell culture medium according to claim 1 . 3 . A method for improving a cell viability, comprising adding a Fe 2+ -OVA compound, wherein a final concentration of Fe 2+ added is 10-100 μM, and a final concentration of OVA added is 10-100 μM; and a cell is a mammalian cell, and the method is for non-diagnostic or non-therapeutic purposes. 4 . The method for improving the cell viability according to claim 3 , wherein the method is performed in an environment being free of an amino acid or deficient in the amino acid. 5 . The method for improving the cell viability according to claim 3 , wherein in the method, an animal serum albumin is replaced with the Fe 2+ -OVA compound.
Assignees
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Classifications
Proteins not provided for elsewhere · CPC title
Serum-free medium, which may still contain naturally-sourced components · CPC title
Iron; Fe chelators; Transferrin · CPC title
Amino acids, peptides or proteins · CPC title
Calcium; Ca chelators; Calcitonin · CPC title
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- What does patent US2025019646A1 cover?
- A cell growth promoter and an application thereof are provided. The invention adopts Fe 2+ and ovalbumin to form a Fe 2+ -ovalbumin (OVA) compound to promote ovalbumin cell endocytosis, the cell growth promoter can maintain cell growth in an environment free of amino acids, promote cell proliferation in an environment with amino acids; and, the Fe 2+ -OVA compound can replace expensive animal …
- Who is the assignee on this patent?
- Univ China Agricultural
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0037. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Jan 16 2025 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).