Data processing module of a biological test system

Patent metadata

Field	Value
Publication number	US-2025014454-A1
Application number	US-202418892734-A
Country	US
Kind code	A1
Filing date	Sep 23, 2024
Priority date	Dec 30, 2019
Publication date	Jan 9, 2025
Grant date	—

Abstract

A test system includes a test container array including a plurality of test containers, and a plurality of electrodes integrated into the test container array. A test container contains a content. The test system further includes a plurality of drive-sense circuits coupled to the plurality of electrodes. When enabled, the set of drive-sense circuits transmit a set of electrode signals on the set of electrodes and generate a set of sensed signals. The test system further includes a processing module coupled to the plurality of drive-sense circuits that includes an analog reference signal generator operable to provide a set of analog reference signals to the set of drive-sense circuits, and a sensed signal processing unit operable to interpret the set of sensed signals as a set of impedance values. The set of impedance values are representative of electrical characteristics of the content.

First claim

What is claimed is:

1 . A test system comprises:
a test container array including a plurality of test containers, wherein a test container of the plurality of test containers is operable to contain a content; a plurality of electrodes integrated into the test container array, wherein a set of electrodes of the plurality of electrodes is integrated into a test container of the test container array; a plurality of drive-sense circuits coupled to the plurality of electrodes, wherein a set of drive-sense circuits of the plurality of drive-sense circuits is coupled to the set of electrodes, and wherein, when enabled, the set of drive-sense circuits are operable to:
transmit a set of electrode signals on the set of electrodes, wherein an electrode signal of the set of electrode signals includes a transmit signal component having a frequency, wherein the electrode signal is based on an analog reference signal including the frequency; and
generate a set of sensed signals, wherein a sensed signal of the set of sensed signals includes at least one frequency component corresponding to at least one transmit signal transmitted by at least one other drive-sense circuit of the set of drive-sense circuits; and
a processing module coupled to the plurality of drive-sense circuits, wherein the processing module includes:
an analog reference signal generator operable to provide a set of analog reference signals to the set of drive-sense circuits; and
a sensed signal processing unit, wherein the sensed signal processing unit is operable to interpret the set of sensed signals as a set of impedance values, wherein the set of impedance values are representative of electrical characteristics of the content.

2 . The test system of claim 1 , wherein the analog reference signal generator further comprises:
a direct current (DC) reference control operable to generate DC voltage components of the set of analog reference signals; and an oscillator control operable to generate alternating current (AC) oscillating components of the set of analog reference signals.

3 . The test system of claim 2 , wherein a drive sense-circuit of the set of drive-sense circuits includes an adder operable to add a DC voltage component of the DC voltage components and an AC oscillating component to produce the analog reference signal.

4 . The test system of claim 2 , wherein the analog reference signal generator further includes a set of adders, wherein an adder of the set of adders is operable to add a DC voltage component of the DC voltage components and an AC oscillating component to produce the analog reference signal.

5 . The test system of claim 1 , wherein the sensed signal processing unit further comprises:
a bandpass filter circuit having a set of bandpass filters coupled to the set of drive-sense circuits, wherein the bandpass filter circuit is operable to convert the set of sensed signals into a set of filtered signals, wherein a bandpass filter of the set of bandpass filters passes signals in a bandpass region corresponding to a frequency component of the at least one frequency component to convert a sensed signal of the set of sensed signals to a filtered signal of the set of filtered signals; and a frequency interpreter coupled to the bandpass filter circuit, wherein the frequency interpreter is operable to:
convert the set of filtered signals into the set of impedance values, wherein the set of impedance values are representative of electrical characteristics of the content.

6 . The test system of claim 1 , wherein the content includes:
a solution; one or more biological cells; a portion of one or more biological cells; and a testing substance.

7 . The test system of claim 1 , wherein the electrical characteristics include one or more of:
position; impedance; size; shape; movement; density; excitability; and potential.

8 . The test system of claim 1 , wherein an impedance value of the set of impedance values includes one or more of:
an actual impedance value; a relative impedance value; and a difference impedance value.

9 . The test system of claim 1 , wherein the processing module is further operable to:
generate an impedance map based on the set of impedance values and with respect to positioning of the set of electrodes, wherein the impedance map is representative of electrical characteristics of the content.

10 . The test system of claim 9 further comprises:
when enabled, and when a second content is added to the test container, the set of drive-sense circuits is operable to:
transmit a second set of electrode signals on the set of electrodes; and
generate a second set of sensed signals; and

the processing module is further operable to:
interpret the second set of sensed signals as a second set of impedance values;
generate a second impedance map based on the second set of impedance values and with respect to the positioning of the set of electrodes, wherein the second impedance map is representative of electrical characteristics of the content and second content; and
compare the impedance map and second impedance map to determine electrical characteristics of the second content.

11 . The test system of claim 10 , wherein the processing module is further operable to:
determine the content is a biological material and the second content is a solution.

12 . The test system of claim 11 , wherein the solution includes one or more of:
a saline solution; a preservative; and a cell culture solution.

13 . The test system of claim 1 , wherein the sensed signal processing unit further comprises:
a set of bandpass filter circuits coupled to the set of drive-sense circuits, wherein a bandpass filter circuit of the set of bandpass filter circuits includes a set of bandpass filters, wherein a bandpass filter circuit of the set of bandpass filter circuits is operable to convert the set of sensed signals to a set of filtered signals, wherein a bandpass filter of the set of bandpass filters passes signals in a bandpass region corresponding to a frequency component of the at least one frequency component to produce a filtered signal of the set of filtered signals; and a set of frequency interpreters coupled to the set of bandpass filter circuits, wherein the set of frequency interpreters is operable to:
convert sets of filtered signals into sets of impedance values, wherein the sets of impedance values are representative of electrical characteristics of the content.

Assignees

• Sigmasense Llc

Inventors

• Van Ostrand Daniel Keith
• Seger Jr Richard Stuart
• Morrison Gerald Dale
• Gray Patrick Troy
• Huynh Phuong
• Markison Timothy W
• Healy Patricia M

Classifications

• G01N33/4836 Primary

  using multielectrode arrays · CPC title

• G01N27/028 Primary

  Circuits therefor (measuring impedance per se G01R27/02) · CPC title

• G01D21/02

  Measuring two or more variables by means not covered by a single other subclass · CPC title

• B01L2300/0663

  Whole sensors · CPC title

• B01L2300/0645

  Electrodes · CPC title