Methods for Monoclonal Antibody Generation
US-2024228595-A1 · Jul 11, 2024 · US
Method for measuring cell concentration
US2024401133A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2024401133-A1 |
| Application number | US-202218697196-A |
| Country | US |
| Kind code | A1 |
| Filing date | Sep 28, 2022 |
| Priority date | Sep 29, 2021 |
| Publication date | Dec 5, 2024 |
| Grant date | — |
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Abstract
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Disclosed is a method for measuring a cell concentration in a sample, the method including the following steps of: (1) measuring the number of copies of a specific gene and a gene of an external standard in a plurality of standard samples each having a known cell concentration by using digital PCR (dPCR); (2) normalizing the measured value of the specific gene measured in the step (1) using the measured value of the external standard and creating a calibration curve on the basis of the normalized value and the known cell concentration; (3) measuring the number of copies of the same specific gene and gene of the external standard in a sample having an unknown cell concentration by using dPCR; and (4) normalizing the measured value of the specific gene measured in the step (3) using the measured value of the external standard and determining a cell concentration using the calibration curve created in the step (2) from the normalized value.
First claim
Opening claim text (preview).
1 . A method for measuring a cell concentration in a sample, the method comprising the following steps of: (1) measuring the number of copies of a specific gene and a gene of an external standard in a plurality of standard samples each having a known cell concentration by using digital PCR (dPCR); (2) normalizing the measured value of the specific gene measured in the step (1) using the measured value of the external standard and creating a calibration curve on the basis of the normalized value and the known cell concentration; (3) measuring the number of copies of the same specific gene and gene of the external standard in a sample having an unknown cell concentration by using dPCR; and (4) normalizing the measured value of the specific gene measured in the step (3) using the measured value of the external standard and determining a cell concentration using the calibration curve created in the step (2) from the normalized value. 2 . The method according to claim 1 , wherein in the step (1), measurement of the number of copies of a specific gene and measurement of the number of copies of a gene of an external standard are simultaneously performed. 3 . The method according to claim 1 , wherein a matrix of each of the standard samples used for creating a calibration curve is different from a matrix of the sample used for measuring a cell concentration. 4 . The method according to claim 1 , wherein accuracy of the value of the cell concentration determined in the step (4) is within ±35%. 5 . The method according to claim 1 , wherein precision of the value of the cell concentration determined in the step (4) is within ±35%. 6 . The method according to claim 1 , wherein the specific gene is LINE-1 gene. 7 . The method according to claim 1 , wherein the specific gene is REXO1L1 gene.
Assignees
Inventors
Classifications
Polymorphic or mutational markers · CPC title
- C12Q1/6876Primary
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title
- C12Q1/6851Primary
Quantitative amplification · CPC title
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- What does patent US2024401133A1 cover?
- Disclosed is a method for measuring a cell concentration in a sample, the method including the following steps of: (1) measuring the number of copies of a specific gene and a gene of an external standard in a plurality of standard samples each having a known cell concentration by using digital PCR (dPCR); (2) normalizing the measured value of the specific gene measured in the step (1) using the…
- Who is the assignee on this patent?
- Takeda Pharmaceuticals Co
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6876. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Dec 05 2024 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).