Method for releasing platelets in turbulent flow and platelet release system for carrying out such method

Patent metadata

Field	Value
Publication number	US-2024360401-A1
Application number	US-202218287728-A
Country	US
Kind code	A1
Filing date	Apr 21, 2022
Priority date	Apr 21, 2021
Publication date	Oct 31, 2024
Grant date	—

Abstract

The disclosure relates to a method for releasing platelets from megakaryocytes contained in a fluid (F), the method being carried out by means of a system comprising two concentric cylinders, an inner cylinder comprising a cylindrical wall and a hollow outer cylinder located radially external to the inner cylinder, the outer cylinder comprising a cylindrical wall of a base at which the second cylinder is closed, the cylinders being separated by a space devoid of any mechanical parts, the space being intended to receive the fluid (F), the method comprising the following steps: supplying the space with a fluid (F) comprising megakaryocytes, rotating the inner cylinder about its axis, the outer cylinder being stationary, or moving the two cylinders in opposite directions about their axes, so as to generate an at least partially turbulent flow of fluid (F) in the space and obtain a second fluid (F′) enriched in platelets.

First claim

1 . A method for releasing platelets from megakaryocytes contained in a fluid (F), the method being implemented by a system comprising two concentric cylinders, an inner cylinder comprising a cylindrical wall and a hollow outer cylinder situated radially external to the inner cylinder, the outer cylinder comprising a cylindrical wall of a base at a level of which the second cylinder is closed, the cylinders being separated by a space devoid of any mechanical part, the space being intended to receive the fluid (F), the method comprising the following steps:
supplying the space with a fluid (F) comprising megakaryocytes, rotating the inner cylinder about its axis, the outer cylinder being stationary, or moving the two cylinders in opposite directions about their axes, so as to generate an at least partially turbulent flow of fluid (F) in the space and obtain a second fluid (F′) enriched with platelets.

2 . The method according to claim 1 , wherein when the inner cylinder is moved while the outer cylinder is stationary, the inner cylinder is moved so as to define a Reynolds number

Re
i

=

r
i

·

ω
i

·
d

ϑ

greater than or equal to 1400, where r i is the radius of the inner cylinder, ω i is the angular speed of the inner cylinder, d is a distance separating the inner cylinder from the outer cylinder and ϑ is the kinematic viscosity of the fluid between the inner and outer cylinders.

3 . The method according to claim 1 , wherein, when the two cylinders are moved in opposite directions, the inner cylinder and the outer cylinder are moved so as to define respectively a Reynolds number

Re
i

=

r
i

·

ω
i

·
d

ϑ

greater than 1000 and a Reynolds number

Re
o

=

r
o

·

ω
o

·
d

ϑ

greater than 1000, where r i is the radius of the inner cylinder, r o is the radius of the outer cylinder, ω i is the angular speed of the inner cylinder, ω o is the angular speed of the outer cylinder, d is a distance separating the inner cylinder from the outer cylinder and ϑ is the kinematic viscosity of the fluid between the inner and outer cylinders.

4 . The method according to claim 1 , wherein the two cylinders are separated by a distance (d) of less than 5 mm.

5 . The method according to claim 1 , wherein the two cylinders are separated by a distance (d) of between 2 mm and 4 mm.

6 . The method according to claim 1 , wherein the two cylinders are separated by a distance (d) of approximately 3 mm.

7 . The method according to claim 1 , wherein the space is continuously supplied with the fluid and the system comprises an inlet for filling the space with the fluid (F) and an opening for evacuating the fluid (F′) enriched with platelets, the opening being located at the level of the base.

8 . The method according to claim 7 , wherein the residence time of the fluid (F) in the space is between 4 minutes and 6 minutes.

9 . The method according to claim 1 , wherein the inner cylinder and the outer cylinder comprise other peripheral internal walls forming a first pattern and a second pattern respectively, the first and second patterns being nested in one another so that the other walls of the inner cylinder and other walls of the outer cylinder are concentric with one another and at least partially opposite one another.

10 . A method for releasing platelets from megakaryocytes contained in a fluid (F), the method being implemented by a system comprising two parallel flat walls separated by a space devoid of any mechanical part, the space being designed to receive the fluid (F), the walls being suitable for movement, the method comprising the following steps:
supplying the space with a fluid (F) comprising megakaryocytes, moving one of the flat walls in a plane of the flat wall, the other of the walls being stationary, or moving the two walls in opposite directions, each wall being moved in its plane, so as to generate an at least partially turbulent flow of fluid (F) in the space and obtain a second fluid (F′) enriched with platelets.

11 . The method according to claim 10 , wherein the first flat wall is the wall of a first conveyor belt and the second flat wall is the wall of a second conveyor belt.

12 . The method according to claim 10 , wherein when the two walls are moving in opposite directions, the two walls are moving in opposite directions at the same speed.

13 . The method according to claim 12 , wherein the two walls move at a speed of approximately 1 metre per second.

14 . The method according to claim 10 , wherein when the two walls move in opposite directions, the two walls move with a speed difference of at most 10% with respect to an average value.

15 . The method according to claim 10 , wherein extracellular vesicles are additionally released.

16 . The method according to claim 7 , wherein the residence time of the fluid (F) in the space is 5 minutes.

Assignees

• Francais Du Sang Ets
• Inst Nat Sante Rech Med
• Univ Strasbourg
• Avignon Univ
• Centre Nat Rech Scient
• Univ Aix Marseille
• Ecole Centrale Marseille

Inventors

• Strassel Catherine
• Mallo Léa
• Do Sacramento Valentin
• Bertrand Eric
• Knapp Yannick

Classifications

• C12M27/10

  Rotating vessel · CPC title

• C12M23/34 Primary

  Internal compartments or partitions · CPC title

• C12M47/04 Primary

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title