Scattering microscopy

Patent metadata

Field	Value
Publication number	US-2024353325-A1
Application number	US-202418761357-A
Country	US
Kind code	A1
Filing date	Jul 2, 2024
Priority date	Mar 21, 2019
Publication date	Oct 24, 2024
Grant date	—

Abstract

A scattering microscopy arrangement uses a microscope to image an object comprising a surface. A light source emits illuminating light and a light detector detects light elastically scattered from the object. An electric potential is applied to the surface that affects the electrochemical properties of the object while imaging. The electric potential provides a contrast mechanism that improves the imaging and allows for characterisation of the object and/or the surrounding environment.

First claim

1 - 20 . (canceled)

21 . A method of monitoring and controlling the binding rate of a particle, the method comprising the steps of;
imaging an object having a surface and at least one particle with a scattering microscope including a light source arranged to emit illuminating light and a light detector, the microscope further arranged to illuminate the object with the illuminating light and to detect light elastically scattered from the object with the light detector; and applying an electrical potential to the surface of the object to change the electrochemical state of the surface; wherein the change of the electrochemical state permits monitoring and controlling of the rate of binding of the particle to the surface, wherein the object is being selected not to have a plasmon resonance frequency at the wavelengths of the illuminating light and at the applied electrical potential.

22 . The method according to claim 21 , wherein an electric double layer forms at the surface and the electrical potential to the surface affects the electrochemical properties of the electric double layer.

23 . The method according to claim 21 , wherein the surface is a surface of a conductive material and the step of applying an electrical potential to the surface comprises applying the potential to the conductive material.

24 . The method according to claim 21 , further comprising the step of selectively rejecting or attracting a particle to the surface by applying electrical potential to the surface.

25 . The method according to claim 21 , further comprising the step of monitoring interactions between the object and constituents in the surrounding environment, wherein the constituents are molecules or ions.

26 . The method according to claim 25 , further comprising the step of adjusting the binding/unbinding rate of molecules in solution to the surface of the object.

27 . The method according to claim 21 , comprising the step of applying a plurality of different electrical potential to the surface.

28 . The method according to claim 27 , further comprising the step of comparing measurements taken at different electrical potentials.

29 . The method according to claim 21 , wherein the object is a single nanoscopic object.

30 . The method according to claim 29 , comprising the step of monitoring the properties and electrochemical state of the single nanoscopic object.

31 . The method according to claim 21 , wherein the particle is a dielectric particle.

32 . The method according to claim 21 , wherein the particle is a protein or an aggregate of proteins, a polypeptide, a glycopolypeptide or glycoprotein, a lipopolypeptide or lipoprotein or any aggregate thereof, a lipid, a proteoglycan, or a sugar polymer.

33 . The method according to claim 21 , wherein the particle may be a nucleic acid molecule, such as an RNA (ribonucleic acid) or DNA (deoxyribonucleic acid), or an artificial nucleic acid molecule, or any aggregate thereof.

34 . The method according to claim 21 , wherein the particle has a mass of 10 kDa or more.

35 . The method according to claim 21 , wherein the particle has a mass of 5000 kDa or less.

Assignees

• Univ Oxford Innovation Ltd

Inventors

• Kukura Philipp
• Faez Sanli

Classifications

• G02B21/361

  Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups) · CPC title

• G02B21/002

  Scanning microscopes (scanning near field optical microscopes G01Q60/18) · CPC title

• G02B21/06 Primary

  Means for illuminating specimens · CPC title

• G01N21/66

  electrically excited, e.g. electroluminescence · CPC title

• G01N21/4795 Primary

  spatially resolved investigating of object in scattering medium (in vivo A61B) · CPC title