Treated cell material used in biological tissue repair

Patent metadata

Field	Value
Publication number	US-2024350711-A1
Application number	US-202218684928-A
Country	US
Kind code	A1
Filing date	Aug 24, 2022
Priority date	Aug 25, 2021
Publication date	Oct 24, 2024
Grant date	—

Abstract

It is desirable to provide a novel biomaterial and the like for tendon repair. Provided is a biomaterial for tendon repair containing a hydrogel formed by means of a cross-linking reaction between a reactive group A and a reactive group B, wherein the hydrogel contains a water-soluble polymer having the reactive group A, and mesenchymal stem cells having the reactive group B.

First claim

1 . A biological material for tendon repair, comprising a hydrogel formed through cross-linking reaction of a reactive group A and a reactive group B, wherein
the hydrogel comprises: a water-soluble polymer having the reactive group A; and mesenchymal stem cells having the reactive group B.

2 . The material according to claim 1 , wherein a combination of the reactive group A and the reactive group B is selected from the group consisting of combinations in the following table:

TABLE 6

Reactive group A
Reactive group B

RG-1
Alkynyl group
Azide group

RG-2
Azide group
Alkynyl group

RG-3
Carboxyl group
Amino group

RG-4
N-Hydroxysuccinimide
Amino group

ester group

RG-5
N-Succinimide group
Amino group

RG-6
Amino group
Carboxyl group

RG-7
Amino group
N-Hydroxysuccinimide

ester group

RG-8
Amino group
N-Succinimide group

RG-9
Sulfhydryl group
Sulfhydryl group

RG-10
Sulfhydryl group
Pyridyl disulfide group

RG-11
Sulfhydryl group
Maleimide group

RG-12
Pyridyl disulfide group
Sulfhydryl group

RG-13
Maleimide group
Sulfhydryl group

3 . The material according to claim 1 , wherein the reactive group A is an alkynyl group, and the reactive group B is an azide group.

4 . The material according to claim 1 , wherein the reactive group A is a cyclic alkyne group, and the reactive group B is an azide group.

5 . The material according to claim 1 , wherein the water-soluble polymer comprises alginic acid bound with a branched compound.

6 . The material according to claim 1 , wherein the water-soluble polymer comprises alginic acid bound with a branched compound selected from the group consisting of the following formulas (1-N), (1-N-1), (1-N-2-1), and (1-N-2-2):

TABLE 7

Formula (1-N)

In formula (1-N), m = 1 to 10, n = 10 to 500, and q = 1 to 10

Formula (1-N-1)

In formula (1-N-1), n 1 = 10-500

Formula (1-N-2-1)

In formula (1-N-2-1), n 2 = 10-500

Formula (1-N-2-2)

7 . The material according to claim 1 , wherein the water-soluble polymer comprises branched alginate (bAlg) represented by the following formula:

wherein X t ═—NHCH 2 CH 2 CH 2 — is a binding pattern in which the imino group is bonded through an amide bond to a carboxyl group of alginate.

8 . The material according to claim 1 , wherein the water-soluble polymer having the reactive group A is cyclooctyne-modified branched alginate (bAlg-DBCO) represented by the following formula:

wherein X 1 ═—NHCH 2 CH 2 CH 2 — is a binding pattern in which the imino group is bonded through an amide bond to a carboxyl group of alginate.

9 . The material according to claim 1 , wherein the mesenchymal stem cells are derived from bone marrow.

10 . The material according to claim 1 , wherein the number of cells contained in the hydrogel is 1.0×10 5 cells/mL or more.

11 . The material according to claim 1 , wherein a concentration of the water-soluble polymer is 0.2% by weight to 8.0% by weight based on the total amount of the hydrogel.

12 . The material according to claim 1 , wherein the hydrogel is an injectable gel.

13 . The material according to claim 1 , wherein the material is suitable for use for the repair of a tendon tissue selected from the group consisting of patella tendon, tibialis anterior tendon, Achilles tendon, popliteus tendon, semitendinosus tendon, gracilis tendon, supraspinatus tendon, infraspinatus tendon, subscapularis tendon, teres minor tendon, rectus femoris tendon, posterior tibial tendon, and quadriceps femoris tendon.

14 . A method for producing a biological material for tendon repair, comprising the s

Assignees

• Mochida Pharm Co Ltd
• Univ Hokkaido Nat Univ Corp
• Konan Gakuen

Inventors

• Iwasaki Norimasa
• Onodera Tomohiro
• Yamaguchi Jun
• Nagahama Koji
• Saito Mitsuru

Classifications

• A61L27/20

  Polysaccharides · CPC title

• A61L2430/10

  for reconstruction of tendons or ligaments · CPC title

• A61L2400/06

  Flowable or injectable implant compositions · CPC title

• A61L27/52

  Hydrogels or hydrocolloids · CPC title

• A61L27/386

  Ligaments, tendons · CPC title