Sample measurement device, sample measurement method, and sample measurement program

Patent metadata

Field	Value
Publication number	US-2024337620-A1
Application number	US-202418749209-A
Country	US
Kind code	A1
Filing date	Jun 20, 2024
Priority date	Jan 27, 2022
Publication date	Oct 10, 2024
Grant date	—

Abstract

A cell culture analysis device comprises a voltage application unit, a current measurement unit, a voltage measurement unit, and an analysis unit. The voltage application unit applies voltage to a sensing unit, which includes a working electrode, a counter electrode, and a reference electrode, of a sensor in a state in which the sensing unit is immersed in the culture medium. The current measurement unit measures the current flowing through the sensor from the voltage applied to the sensing unit. The analysis unit calculates the concentration of glucose or the like contained in a culture medium based on the measurement result. The voltage measurement unit measures the terminal voltage of the counter electrode in a state in which the voltage application unit is applying voltage. The analysis unit detects a measurement error based on the terminal voltage of the counter electrode.

First claim

1 . A sample measurement device, comprising:
a voltage application unit configured to apply voltage to an electrode unit including at least a working electrode, a counter electrode, and a reference electrode of a sample measurement sensor in a state in which the electrode unit is immersed in a sample; a current measurement unit configured to measure a current flowing through the sample measurement sensor by the voltage applied to the electrode unit; a concentration measurement unit configured to calculate a concentration of an analyte contained in the sample on the basis of a measurement result from the current measurement unit; a counter electrode terminal voltage measurement unit configured to measure a terminal voltage of the counter electrode included in the electrode unit in a state in which a voltage is being applied by the voltage application unit; and a measurement error detection unit configured to detect a measurement error on the basis of the terminal voltage of the counter electrode measured by the counter electrode terminal voltage measurement unit.

2 . The sample measurement device according to claim 1 ,
wherein the measurement error detection unit detects a contamination error attributable to an admixture of an impurity other than the sample.

3 . The sample measurement device according to claim 2 ,
wherein the measurement error detection unit determines that there is a contamination error when a fluctuation rate of the measurement result in the counter electrode terminal voltage measurement unit is at or below a specific threshold.

4 . The sample measurement device according to claim 3 ,
wherein the measurement error detection unit sets a skip period in which the measurement is not performed until a specific time has elapsed since the voltage was applied from the voltage application unit, and it is determined whether or not there is a contamination error after the skip period has elapsed.

5 . The sample measurement device according to claim 1 ,
wherein the measurement error detection unit detects an improper immersion error caused by improper immersion in which the electrode unit is not properly immersed in the sample.

6 . The sample measurement device according to claim 5 ,
wherein the measurement error detection unit sets a skip period in which the measurement is not performed until a specific time has elapsed since voltage was applied from the voltage application unit.

7 . The sample measurement device according to claim 6 ,
wherein the measurement error detection unit determines that there is an improper immersion error when the terminal voltage of the counter electrode sensed during the skip period is outside a specific range.

8 . The sample measurement device according to claim 7 ,
wherein the voltage application unit stops an application of voltage when it is determined that there is an improper immersion error.

9 . The sample measurement device according to claim 1 ,
further comprising a display unit configured to display a detection result for the measurement error in the measurement error detection unit.

10 . The sample measurement device according to claim 1 ,
wherein the sample measurement sensor comprises the electrode unit including at least the working electrode, the counter electrode, and the reference electrode, in the working electrode, a reagent applied to a surface of the electrode unit is covered with a protective film, in the reference electrode, the surface of the electrode unit is covered with a protective film, and in the counter electrode, an electrode portion is exposed to the sample.

11 . The sample measurement device according to claim 10 ,
wherein at least one of the working electrode and the reference is such that the surface of the electrode portion is formed in a substantially circular shape in plan view.

12 . The sample measurement device according to claim 10 ,
wherein in the counter electrode, the surface of the electrode unit is formed in a substantially rectangular shape in plan view.

13 . The sample measurement device according to claim 10 ,
wherein the electrode portion of the counter electrode is formed from carbon.

14 . A sample measurement method, comprising:
a voltage application step of applying voltage to an electrode unit in a state in which the electrode unit including at least a working electrode, a counter electrode, and a reference electrode of a sample measurement sensor is immersed in a sample; a current measurement step of measuring a current flowing through the sample measurement sensor by the voltage applied in the voltage application step; a concentration measurement step of calculating a concentration of an analyte contained in the sample on the basis of a measurement result in the current measurement step; a counter electrode terminal voltage measurement step of measuring a terminal voltage of the counter electrode included in the electrode unit in a state in which voltage is being applied in the voltage applying step; and a measurement error detection step of detecting a measurement error on the basis of the terminal voltage of the counter electrode measured in the counter electrode terminal voltage measurement step.

15 . A sample measurement program that causes a computer to execute a sample measurement method comprising:
a voltage application step of applying voltage to an electrode unit in a state in which the electrode unit including at least a working electrode, a counter electrode, and a reference electrode of a sample measurement sensor is immersed in a sample; a current measurement step of measuring a current flowing through the sample measurement sensor by the voltage applied in the voltage application step; a concentration measurement step of calculating a concentration of an analyte contained in the sample on the basis of a measurement result in the current measurement step; a counter electrode terminal voltage measurement step of measuring a terminal voltage of the counter electrode included in the electrode unit in a state in which voltage is being applied in the voltage applying step; and a measurement error detection step of detecting a measurement error on the basis of the terminal voltage of the counter electrode measured in the counter electrode terminal voltage measurement step.

Assignees

• Phc Holdings Corp

Inventors

• Iketani Seiitirou
• Fujita Sumitaka
• Fukumoto Satoshi

Classifications

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• A61B5/14532

  for measuring glucose, e.g. by tissue impedance measurement · CPC title

• G01N27/3274 Primary

  Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration (coding of calibration information G01N33/48771) · CPC title

• G01N27/38

  Cleaning of electrodes · CPC title

• G01N27/308

  at least partially made of carbon · CPC title