Cell culture membrane and method for producing biological tissue

Patent metadata

Field	Value
Publication number	US-2024327770-A1
Application number	US-202418675517-A
Country	US
Kind code	A1
Filing date	May 28, 2024
Priority date	Dec 22, 2021
Publication date	Oct 3, 2024
Grant date	—

Abstract

The disclosure makes it possible to provide a cell culture membrane capable of easily decomposing after allowing cell layers to be formed on both sides of the cell culture membrane. A cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane containing methyl cellulose having a weight average molecular weight of from 20000 to 190000 as a main component, or containing methyl cellulose having a weight average molecular weight of from 190000 to 400000 as a main component, and further containing a dissolution temperature regulator. The dissolution temperature regulator is preferably at least one of sodium styrenesulfonate, sodium iodide, or sodium toluenesulfonate. A poorly soluble material is preferably added to the cell culture membrane.

First claim

1 . A cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane comprising methyl cellulose having a weight average molecular weight of from 20000 to 190000 as a main component.

2 . A cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane comprising methyl cellulose having a weight average molecular weight of from 190000 to 400000 as a main component, and further comprising a dissolution temperature regulator.

3 . The cell culture membrane according to claim 2 , wherein the dissolution temperature regulator is at least one of sodium styrenesulfonate, sodium iodide, or sodium toluenesulfonate.

4 . The cell culture membrane according to claim 1 , wherein a poorly soluble material is added to the cell culture membrane.

5 . The cell culture membrane according to claim 4 , wherein the poorly soluble material is at least one of polyethylene terephthalate, a glass fiber, a cellulose ester, polyvinylidene fluoride, or polycarbonate.

6 . The cell culture membrane according to claim 4 , wherein the poorly soluble material is at least one of polystyrene, a cycloolefin polymer, or a cycloolefin copolymer.

7 . The cell culture membrane according to claim 6 , wherein the cell culture membrane has a scaffold material for cells on at least one surface thereof.

8 . The cell culture membrane according to claim 7 , wherein the scaffold material is at least one of collagen, Matrigel, fibronectin, laminin, chitosan, or silk.

9 . The cell culture membrane according to claim 6 , wherein at least one surface of the cell culture membrane is subjected to surface treatment.

10 . The cell culture membrane according to claim 1 , further comprising adhesive cells.

11 . A method for producing a biological tissue including a plurality of cell layers, the method comprising:
supplying cells and a culture solution to two culture spaces of a biological tissue forming device including a cell culture membrane and the two culture spaces, the cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane being disposed between a plurality of cell layers after the cells are cultured, and the cell culture membrane further containing methyl cellulose having a weight average molecular weight of from 20000 to 190000 as a main component, and the two culture spaces being divided by the cell culture membrane; culturing the cells in the two culture spaces to form cell layers on both surfaces of the cell culture membrane; and dissolving methyl cellulose in the cell culture membrane at 15° C. or lower.

12 . A method for producing a biological tissue including a plurality of cell layers, the method comprising:
supplying cells and a culture solution to two culture spaces of a biological tissue forming device including a cell culture membrane and the two culture spaces, the cell culture membrane having a cell culture region at both surfaces thereof, the cell culture membrane being disposed between a plurality of cell layers after the cells are cultured, the cell culture membrane containing methyl cellulose having a weight average molecular weight of from 190000 to 400000 as a main component, and the cell culture membrane further containing a dissolution temperature regulator, and the two culture spaces being divided by the cell culture membrane; culturing the cells in the two culture spaces to form cell layers on both surfaces of the cell culture membrane; and dissolving methyl cellulose in the cell culture membrane at 20° C. or lower.

13 . The method for producing a biological tissue according to claim 11 , wherein methyl cellulose in the cell culture membrane is dissolved in 10 minutes or less.

14 . The method for producing a biological tissue according to claim 11 , wherein a poorly soluble material is added to the cell culture membrane, and the cell layers are supported by the poorly soluble material after dissolution of methyl cellulose.

Assignees

• Toyo Seikan Group Holdings Ltd

Inventors

• Otsuki Azusa
• Kuninori Masahiro
• Ichikawa Kentarou

Classifications

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M23/20

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C08L1/02

  Cellulose; Modified cellulose · CPC title

• C12N5/0062

  General methods for three-dimensional culture · CPC title